Walker Jennifer M, Klakotskaia Diana, Ajit Deepa, Weisman Gary A, Wood W Gibson, Sun Grace Y, Serfozo Peter, Simonyi Agnes, Schachtman Todd R
Department of Psychological Sciences, University of Missouri, Columbia, MO, USA.
Department of Biochemistry, University of Missouri, Columbia, MO, USA Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO, USA.
J Alzheimers Dis. 2015;44(2):561-72. doi: 10.3233/JAD-140981.
Alzheimer's disease (AD) is a progressive, age-dependent neurodegenerative disorder affecting specific brain regions that control memory and cognitive functions. Epidemiological studies suggest that exercise and dietary antioxidants are beneficial in reducing AD risk. To date, botanical flavonoids are consistently associated with the prevention of age-related diseases. The present study investigated the effects of 4 months of wheel-running exercise, initiated at 2-months of age, in conjunction with the effects of the green tea catechin (-)-epigallocatechin-3-gallate (EGCG) administered orally in the drinking water (50 mg/kg daily) on: (1) behavioral measures: learning and memory performance in the Barnes maze, nest building, open-field, anxiety in the light-dark box; and (2) soluble amyloid-β (Aβ) levels in the cortex and hippocampus in TgCRND8 (Tg) mice. Untreated Tg mice showed hyperactivity, relatively poor nest building behaviors, and deficits in spatial learning in the Barnes maze. Both EGCG and voluntary exercise, separately and in combination, were able to attenuate nest building and Barnes maze performance deficits. Additionally, these interventions lowered soluble Aβ1-42 levels in the cortex and hippocampus. These results, together with epidemiological and clinical studies in humans, suggest that dietary polyphenols and exercise may have beneficial effects on brain health and slow the progression of AD.
阿尔茨海默病（AD）是一种进行性、年龄依赖性神经退行性疾病，会影响控制记忆和认知功能的特定脑区。流行病学研究表明，运动和膳食抗氧化剂有助于降低患AD的风险。迄今为止，植物类黄酮一直与预防年龄相关疾病有关。本研究调查了从2月龄开始的4个月轮转运动的效果，以及在饮用水中口服绿茶儿茶素（-）-表没食子儿茶素-3-没食子酸酯（EGCG，每日50毫克/千克）的效果，具体涉及：（1）行为指标：在巴恩斯迷宫中的学习和记忆表现、筑巢行为、旷场实验、明暗箱中的焦虑情况；（2）TgCRND8（Tg）小鼠大脑皮层和海马体中可溶性淀粉样β蛋白（Aβ）的水平。未经治疗的Tg小鼠表现出多动、筑巢行为相对较差以及在巴恩斯迷宫中空间学习能力不足。EGCG和自愿运动单独或联合使用均能够减轻筑巢行为和巴恩斯迷宫表现方面的缺陷。此外，这些干预措施降低了大脑皮层和海马体中可溶性Aβ1-42的水平。这些结果与人类的流行病学和临床研究一起表明，膳食多酚和运动可能对大脑健康有益，并减缓AD的进展。
