Ticak Tomislav, Hariraju Dinesh, Arcelay Margarette Bayron, Arivett Brock A, Fiester Steven E, Ferguson Donald J
Department of Microbiology, Miami University, 700 East High Street, Oxford, OH, 45056, USA.
Arch Microbiol. 2015 Mar;197(2):197-209. doi: 10.1007/s00203-014-1043-6. Epub 2014 Oct 16.
Two novel strains of methanogens were isolated from an estuarine sediment with the capability to utilize quaternary amines. Based on the 16S rRNA analysis, strain B1d shared 99 % sequence identity with Methanolobus vulcani PL-12/M(T) and strain Q3c shared 99 % identity with Methanococcoides sp. PM1 and PM2, but our current isolates display clearly different capabilities of growth on quaternary amines and were isolated based on these capabilities. Strain Q3c was capable of growth on tetramethylammonium and choline, while strain B1d was capable of growth on glycine betaine. Ml. vulcani PL-12/M(T) was incapable of growth on glycine betaine, indicating an obvious distinction between strains B1d and PL-12/M(T). Strain Q3c now represents the only known tetramethylammonium-utilizing methanogen in isolation. Strain B1d is the first quaternary amine-utilizing methanogen from the genus Methanolobus. This study suggests that quaternary amines may serve as ready precursors of biological methane production in marine environments.
从河口沉积物中分离出两种新型产甲烷菌菌株，它们具有利用季胺的能力。基于16S rRNA分析，菌株B1d与火山甲醇叶菌PL-12/M(T)的序列同一性为99%，菌株Q3c与甲烷球菌属PM1和PM2的同一性为99%，但我们目前分离出的菌株在季胺上的生长能力明显不同，并且是基于这些能力分离得到的。菌株Q3c能够在四甲基铵和胆碱上生长，而菌株B1d能够在甘氨酸甜菜碱上生长。火山甲醇叶菌PL-12/M(T)不能在甘氨酸甜菜碱上生长，这表明菌株B1d和PL-12/M(T)之间存在明显差异。菌株Q3c是目前唯一已知的分离出来的利用四甲基铵的产甲烷菌。菌株B1d是甲醇叶菌属中首个利用季胺的产甲烷菌。这项研究表明，季胺可能是海洋环境中生物甲烷产生的现成前体。
