Microbiology Section, Directorate of Groundnut Research (ICAR), Ivnagar Road, PB No. 5, Junagadh, Gujarat 362001 India.
Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012 India.
Indian J Microbiol. 2014 Dec;54(4):471-3. doi: 10.1007/s12088-014-0483-7. Epub 2014 Jul 10.
Haloarchaea are predominant in the salt crystallizers of the Rann of Kutch when the concentration of salts approaches saturation levels. The obligate and extreme halophilic archaeon 3A1-DGR, isolated from a salt crystallizer pond of the Little Rann of Kutch, India, needs minimum of 10 % NaCl in the growth medium. To understand the mechanism(s) of osmotolerance and adaptation at extreme osmolarity, and to mine relevant gene(s), the genome of this haloarchaeon, 3A1-DGR, was sequenced. We report here, the 2.88 Mb draft genome sequence of the haloarchaeon 3A1-DGR, with G+C content of 68 % and the possible involvement of 43 genes in stress tolerance. Further studies of the genome of this haloarchaeon would be required to identify gene(s) that might be responsible for imparting extreme osmotolerance.
当盐浓度接近饱和水平时,盐晶器中的嗜盐古菌占主导地位。从印度小卡奇盐沼盐晶器池塘中分离到的专性极端嗜盐古菌 3A1-DGR 在生长培养基中需要最低 10%的 NaCl。为了了解耐渗透压和适应极端渗透压的机制,并挖掘相关基因,对该嗜盐古菌的基因组进行了测序。我们在此报告,2.88 Mb 嗜盐古菌 3A1-DGR 的草图基因组序列,GC 含量为 68%,可能有 43 个基因参与应激耐受。需要进一步研究该嗜盐古菌的基因组,以确定可能导致其具有极端耐渗透压的基因。