Kıvanç Sertaç Argun, Takım Murat, Kıvanç Merih, Güllülü Gülay
Oltu Public Hospital, Eye Diseases Clinic, Oltu, Erzurum, Turkey.
Anadolu University, Faculty of Science, Department of Biology, Eskisehir,Turkey.
Afr Health Sci. 2014 Jun;14(2):364-71. doi: 10.4314/ahs.v14i2.11.
In this study, we attempted to screen and investigate antibacterial activity of Bacillus species, which were isolated from conjunctiva, against other eyes pathogens.
To examine predominant isolates of Bacillus subtilis, B. pumilus, B. cereus and B. mojevensis, isolated from conjunctiva for their antimicrobial activity against indicator microorganisms as Micrococcus luteus, Staphyloccocus aureus, S. epidermidis, S.hominis, S. lugdunensis, S.warneri, S. haemolyticus, B. cereus, Listeria monocytogenes, and Proteus mirabilis. Growth inhibitions of indicator microorganisms were tested using agar diffusion tests by cells and supernatants of five B. mojevensis, one B. subtilis, four B. cereus and five B. pumilus strains which were isolated from conjunctiva.
The Bacillus isolates showed variable ability of inhibition against the tested microorganisms. Two strains of B. pumillus, 1 strain of B. subtilis, 5 strains of B. mojevensis, 1 strain of B. cereus were efficacious against the tested microorganisms. Most resistant microorganism to these bacteria was Proteus mirabilis. Two of Gram positive bacteria, S. lugdenensis (K15-9) and S. aureus (SDA48), were also found as resistant.
In this study, Bacillus spp isolated from conjunctiva showed antimicrobial activity against Gram-positive bacteria. Human eye-derived microorganisms and their antimicrobial effects might be a useful source of natural products for the future.
在本研究中，我们试图筛选并研究从结膜分离出的芽孢杆菌属物种对其他眼部病原体的抗菌活性。
为检测从结膜分离出的枯草芽孢杆菌、短小芽孢杆菌、蜡样芽孢杆菌和莫吉廖夫芽孢杆菌的主要分离株对指示微生物（如藤黄微球菌、金黄色葡萄球菌、表皮葡萄球菌、人葡萄球菌、路邓葡萄球菌、沃纳葡萄球菌、溶血葡萄球菌、蜡样芽孢杆菌、单核细胞增生李斯特菌和奇异变形杆菌）的抗菌活性。使用琼脂扩散试验，通过从结膜分离出的5株莫吉廖夫芽孢杆菌、1株枯草芽孢杆菌、4株蜡样芽孢杆菌和5株短小芽孢杆菌的细胞和上清液，检测指示微生物的生长抑制情况。
芽孢杆菌分离株对受试微生物的抑制能力各不相同。2株短小芽孢杆菌、1株枯草芽孢杆菌、5株莫吉廖夫芽孢杆菌、1株蜡样芽孢杆菌对受试微生物有效。对这些细菌最具抗性的微生物是奇异变形杆菌。还发现2种革兰氏阳性菌，即路邓葡萄球菌（K15 - 9）和金黄色葡萄球菌（SDA48）具有抗性。
在本研究中，从结膜分离出的芽孢杆菌属对革兰氏阳性菌具有抗菌活性。人眼来源的微生物及其抗菌作用可能是未来天然产物的一个有用来源。
