Gossa Selamawit, Nayak Debasis, Zinselmeyer Bernd H, McGavern Dorian B
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Sci Rep. 2014 Oct 17;4:6664. doi: 10.1038/srep06664.
Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivo imaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivo two-photon imaging.
荧光蛋白（FPs）组合常用于多参数体内成像实验，以可视化标记蛋白或感兴趣的细胞群体。涉及荧光蛋白的研究常常受到光谱重叠、毒性、相对量子效率以及转移到非耐受受体后产生免疫排斥可能性的限制。在这里，我们评估了几种常用荧光蛋白在小鼠免疫系统中的免疫可见性，并确定了一种光谱兼容、免疫耐受的荧光蛋白组合，非常适合用于体内双光子成像。
