Palac R T, Gray L L, Turner F E, Brown P H, Malinow M R, Demots H
Department of Medicine, Oregon Health Sciences University, Portland.
Nucl Med Commun. 1989 Nov;10(11):841-50. doi: 10.1097/00006231-198911000-00009.
It has been observed that atherosclerotic lesions (AL) concentrate certain porphyrins. We evaluated the usefulness of 111In-labelled Photofrin II (PFII), a porphyrin derived from haematoporphyrin derivative, for detection of experimental AL in cholesterol fed rabbits. Three groups of rabbits were studied: non-atherosclerotic (n = 3), 6 and 12 week cholesterol fed (n = 4, 3). 111In-PFII was injected intravenously and gamma camera images were obtained at 24 and 48 h. At 48 h, explanted aortas were also imaged. Aortic arch (AA) to background (BKG) count ratios were calculated from images of the whole body and explanted aortas. AA/BKG ratios were significantly higher in the 12 week cholesterol fed rabbits (3.9 +/- 0.72 at 24 h) and (4.0 +/- 0.67 at 48 h) than in the non-atherosclerotic rabbits (2.2 +/- 0.07 at 24 h) and (2.3 +/- 0.18 at 48 h) (p less than 0.05). The AA/BKG ratio for the explanted aortas showed similar results. Additionally, in two of three 12 week cholesterol fed rabbits, focal count deposition was visible on the whole animal images at the site of aortic arch atherosclerosis. We conclude that 111In-PFII concentrates in AL as early as 24 h after injection and has the potential to be used as an imaging agent for experimental atherosclerosis.
据观察，动脉粥样硬化病变（AL）会聚集某些卟啉。我们评估了111铟标记的卟吩姆钠（PFII）（一种源自血卟啉衍生物的卟啉）对检测喂食胆固醇的兔实验性动脉粥样硬化病变的效用。研究了三组兔子：非动脉粥样硬化组（n = 3）、喂食胆固醇6周和12周组（n = 4、3）。静脉注射111铟 - PFII，并在24小时和48小时获取γ相机图像。在48小时时，还对取出的主动脉进行成像。从全身图像和取出的主动脉图像计算主动脉弓（AA）与本底（BKG）的计数比。喂食胆固醇12周的兔子的AA/BKG比（24小时时为3.9±0.72，48小时时为4.0±0.67）显著高于非动脉粥样硬化兔子（24小时时为2.2±0.07，48小时时为2.3±0.18）（p<0.05）。取出的主动脉的AA/BKG比显示出类似结果。此外，在三只喂食胆固醇12周的兔子中的两只中，在主动脉弓动脉粥样硬化部位的全动物图像上可见局灶性计数沉积。我们得出结论，111铟 - PFII在注射后24小时就会聚集在动脉粥样硬化病变中，有潜力用作实验性动脉粥样硬化的成像剂。
