Yang You-Jian, Zhang Yan-Ting, Chen Guo-Qiang, Cheng Dan, Qiu Ji-Guo, He Qin, He Jian
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou, Jiangsu 215021, PR China.
Int J Syst Evol Microbiol. 2018 Jan;68(1):211-216. doi: 10.1099/ijsem.0.002484. Epub 2017 Nov 14.
A bacterial strain designated YYJ7-1 was isolated from farmland soil in China and characterized using a polyphasic taxonomic approach. Cells of strain YYJ7-1 were Gram-staining-positive, aerobic or facultatively anaerobic, rod-shaped, motile and endospore-forming. Growth occurred at 18-42 °C (optimum at 35 °C), at pH 6.0-8.0 (optimum at pH 7.5) and with 0.0-4.0 % NaCl (optimum with 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus and showed high levels of sequence similarity with respect to Paenibacillus provencensis 4401170 (98.6 %) and Paenibacillus urinalis 5402403 (98.4 %), while lower 16S rRNA gene sequence similarities were observed with all other type strains (97.0 %). However, strain YYJ7-1 showed low DNA-DNA relatedness with P. provencensis 4401170 48.7±4.5 % (43.6±7.1 % in a reciprocal experiment), and P. urinalis 5402403 38.9±5.7 % (35.6±6.8 %). The major cellular fatty acids (>10.0 %) of strain YYJ7-1 were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The polar lipid profile consisted of phospholipids, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major isoprenoid quinone was MK-7. The DNA G+C content was 39.4 mol%. Based on these results, it is concluded that strain YYJ7-1 represents a novel species of the genus Paenibacillus, for which the name Paenibacillus shunpengii sp. nov. is proposed, with YYJ7-1 (=ACCC 19965=KCTC 33849) as the type strain.
从中国农田土壤中分离出一株编号为YYJ7-1的细菌菌株，并采用多相分类法对其进行了鉴定。YYJ7-1菌株细胞革兰氏染色呈阳性，好氧或兼性厌氧，杆状，具运动性，能形成芽孢。该菌株在18-42 °C（最适温度35 °C）、pH 6.0-8.0（最适pH 7.5）和0.0-4.0 % NaCl（最适0.5 %）条件下生长。基于16S rRNA基因序列的系统发育分析表明，该菌株属于芽孢杆菌属，与普罗旺斯芽孢杆菌4401170（98.6 %）和尿液芽孢杆菌5402403（98.4 %）的序列相似性较高，而与所有其他模式菌株的16S rRNA基因序列相似性较低（97.0 %）。然而，YYJ7-1菌株与普罗旺斯芽孢杆菌4401170的DNA-DNA相关性较低，为48.7±4.5 %（反向实验中为43.6±7.1 %），与尿液芽孢杆菌5402403的DNA-DNA相关性为38.9±5.7 %（35.6±6.8 %）。YYJ7-1菌株的主要细胞脂肪酸（>10.0 %）为anteiso-C15 : 0、iso-C16 : 0和anteiso-C17 : 0。极性脂质谱由磷脂、双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。主要的类异戊二烯醌为MK-7。DNA G+C含量为39.4 mol%。基于这些结果，得出结论：YYJ7-1菌株代表芽孢杆菌属的一个新物种，为此提出新名称顺鹏芽孢杆菌（Paenibacillus shunpengii sp. nov.），以YYJ7-1（=ACCC 19965=KCTC 33849）作为模式菌株。
