Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
College of Life Science, Xinjiang Normal University, Urumqi, XinJiang, 830046, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2690-2695. doi: 10.1099/ijsem.0.004093. Epub 2020 Apr 15.
A Gram-stain-positive, motile, rod-shaped bacterium, designated strain LAM7113, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, PR China. Strain LAM7113 grew optimally at pH 8.0, 30 °C and in the presence of 1.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LAM7113 was closely related to members of the genus , with the highest similarity to Back-11 (96.2 %). The genomic DNA G+C content was 43.4 mol%. The values of average nucleotide identity and DNA-DNA hybridization were 66.1 and 27.0 %, respectively, by comparing the draft genome sequences of strain LAM7113 and Back-11. Anteiso-C and iso-C were identified as the major cellular fatty acids. Menaquinone-7 was detected as the predominant respiratory quinone. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified aminophospholipids, three unidentified glycolipids, one unidentified phospholipid and two unknown polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7113 is proposed to represent a novel species of the genus with the name sp. nov. The type strain is LAM7113 (=CGMCC 1.16619=JCM 32513).
一株革兰氏阳性、可运动、杆状的细菌,被命名为 LAM7113 菌株,是从中国新疆维吾尔自治区桦树林土壤样本中分离得到的。LAM7113 菌株在 pH8.0、30°C 和 1.0%(w/v)NaCl 存在下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明,LAM7113 菌株与属内的成员密切相关,与 Back-11 (96.2%)最为相似。基因组 DNA G+C 含量为 43.4 mol%。通过比较 LAM7113 菌株和 Back-11 的基因组草图序列,发现平均核苷酸同一性和 DNA-DNA 杂交值分别为 66.1%和 27.0%。细胞脂肪酸主要由 anteiso-C 和 iso-C 组成。menaquinone-7 被鉴定为主要的呼吸醌。主要的极性脂类有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、三种未鉴定的氨基磷脂、三种未鉴定的糖脂、一种未鉴定的磷脂和两种未知的极性脂类。基于其表型、系统发育和化学分类学特征,LAM7113 菌株被提议代表属的一个新种,命名为 sp. nov. 。模式菌株为 LAM7113 (=CGMCC 1.16619=JCM 32513)。