Wang Rui, Hong Jie, Liu Ruixin, Chen Maopei, Xu Min, Gu Wiqiong, Zhang Yifei, Ma Qinyun, Wang Feng, Shi Juan, Wang Jiqiu, Wang Weiqing, Ning Guang
Shanghai Clinical Center for Endocrine and Metabolic DiseasesShanghai Institute of Endocrine and Metabolic Diseases, Department of Endocrinology and Metabolism, Shanghai Key Laboratory for Endocrine Tumors and E-Institutes of Shanghai Universities, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, ChinaLaboratory for Endocrine and MetabolismInstitute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Shanghai Clinical Center for Endocrine and Metabolic DiseasesShanghai Institute of Endocrine and Metabolic Diseases, Department of Endocrinology and Metabolism, Shanghai Key Laboratory for Endocrine Tumors and E-Institutes of Shanghai Universities, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, ChinaLaboratory for Endocrine and MetabolismInstitute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
J Mol Endocrinol. 2014 Dec;53(3):405-15. doi: 10.1530/JME-14-0037. Epub 2014 Oct 16.
WNT/β-catenin signalling is involved in regulating adipogenesis, and its dysregulation occurs in obesity. Secreted frizzled-related protein 5 (SFRP5) is a WNT protein inhibitor; however, its role in adipogenesis and obesity is controversial. In this study, we observed that SFRP5 mRNA levels were increased in the fat tissues of obese humans and mice. Sfrp5 expression was gradually induced during differentiation of white and brown adipocytes and was highly increased in mature adipocytes rather than preadipocytes. However, the effects of the exogenous overexpression of Sfrp5 indicated that Sfrp5 may not directly regulate adipogenesis in vitro under the conditions studied. Moreover, SFRP5 did not inhibit the canonical WNT/β-catenin signalling pathway in preadipocytes. Subsequently, we measured the levels of circulating SFRP5 in obese patients and non-obese subjects using ELISA and did not find any significant difference. Collectively, these findings indicate that Sfrp5 represents a candidate for a mature adipocyte marker gene. Our data provide new evidence concerning the role of SFRP5 in adipogenesis of white and brown adipocytes and obesity.
WNT/β-连环蛋白信号通路参与调节脂肪生成，其失调在肥胖症中出现。分泌型卷曲相关蛋白5（SFRP5）是一种WNT蛋白抑制剂；然而，其在脂肪生成和肥胖症中的作用存在争议。在本研究中，我们观察到肥胖人类和小鼠的脂肪组织中SFRP5 mRNA水平升高。在白色和棕色脂肪细胞分化过程中，Sfrp5表达逐渐被诱导，并且在成熟脂肪细胞中高度增加，而在前脂肪细胞中则不然。然而，Sfrp5外源性过表达的结果表明，在所研究的条件下，Sfrp5在体外可能不会直接调节脂肪生成。此外，SFRP5在前脂肪细胞中并未抑制经典的WNT/β-连环蛋白信号通路。随后，我们使用酶联免疫吸附测定法（ELISA）测量了肥胖患者和非肥胖受试者的循环SFRP5水平，未发现任何显著差异。总体而言，这些发现表明Sfrp5是成熟脂肪细胞标记基因的一个候选基因。我们的数据为SFRP5在白色和棕色脂肪细胞脂肪生成及肥胖症中的作用提供了新证据。
