Department of Family Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 81342, Taiwan.
Division of Colon and Rectal Surgery, Department of Surgery, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City 23142, Taiwan.
Cells. 2022 Mar 22;11(7):1065. doi: 10.3390/cells11071065.
Long noncoding RNAs play a key role in the progression of colorectal cancer (CRC). However, the role and mechanism of LOC550643 in CRC cell growth and metastasis remain largely unknown. In this study, we assessed the clinical impacts of LOC550643 on CRC through the analysis of The Cancer Genome Atlas database, which revealed the significant upregulation of LOC550643 in CRC. Moreover, the high expression of LOC550643 was associated with poor survival in patients with CRC ( = 0.001). Multivariate Cox regression analysis indicated that LOC550643 overexpression was an independent prognostic factor for shorter overall survival in patients with CRC (adjusted hazard ratio, 1.90; 95% confidence interval, 1.21-3.00; = 0.006). A biological function analysis revealed that LOC550643 knockdown reduced colon cancer cell growth by hindering cell cycle progression. In addition, LOC550643 knockdown significantly induced cell apoptosis through the inhibition of signaling activity in phosphoinositide 3-kinases. Moreover, LOC550643 knockdown contributed to the inhibition of migration and invasion ability in colon cancer cells. Furthermore, miR-29b-2-5p interacted with the LOC550643 sequence. Ectopic miR-29b-2-5p significantly suppressed colon cancer cell growth and motility and induced cell apoptosis. Our findings suggest that, LOC550643-miR-29b-2-5p axis was determined to participate in the growth and metastasis of colon cancer cells; this could serve as a useful molecular biomarker for cancer diagnosis and as a potential therapeutic target for CRC.
长链非编码 RNA 在结直肠癌（CRC）的进展中发挥着关键作用。然而，LOC550643 在 CRC 细胞生长和转移中的作用和机制在很大程度上仍是未知的。在这项研究中，我们通过分析癌症基因组图谱数据库评估了 LOC550643 对 CRC 的临床影响，该数据库显示 LOC550643 在 CRC 中显著上调。此外，LOC550643 的高表达与 CRC 患者的不良生存相关（ = 0.001）。多变量 Cox 回归分析表明，LOC550643 过表达是 CRC 患者总生存期较短的独立预后因素（调整后的危险比，1.90；95%置信区间，1.21-3.00； = 0.006）。生物学功能分析表明，LOC550643 敲低通过抑制细胞周期进程来降低结肠癌细胞生长。此外，LOC550643 敲低通过抑制磷酸肌醇 3-激酶信号活性显著诱导细胞凋亡。此外，LOC550643 敲低有助于抑制结肠癌细胞的迁移和侵袭能力。此外，miR-29b-2-5p 与 LOC550643 序列相互作用。外源性 miR-29b-2-5p 显著抑制结肠癌细胞的生长和运动，并诱导细胞凋亡。我们的研究结果表明，LOC550643-miR-29b-2-5p 轴被确定参与了结肠癌细胞的生长和转移；这可以作为癌症诊断的有用分子生物标志物，并作为 CRC 的潜在治疗靶点。
