Mirea Cecil Sorin, Schenker Michael, Petre-Mandache Bianca, Cucu Mihai-Gabriel, Camen Georgiana-Cristiana, Vîlcea Ionică Daniel, Albu Bogdan Cristian, Obleagă Cosmin Vasile, Ciorbagiu Mihai Călin, Streață Ioana, Pleșea Răzvan Mihail, Riza Anca-Lelia, Burada Florin
Department of Surgery, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.
Department of Surgery, Emergency Clinical County Hospital, 200642 Craiova, Romania.
Int J Mol Sci. 2025 Mar 27;26(7):3078. doi: 10.3390/ijms26073078.
Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality, influenced by both genetic and epigenetic factors. Long non-coding RNAs (lncRNAs) such as GAS5 and CASC8 have been implicated in cancer susceptibility. This study aimed to assess the association of GAS5 rs145204276 ins/del and CASC8 rs10505477 A>G polymorphisms with CRC risk in a Romanian population. A case-control study was conducted, including 156 CRC patients and 195 healthy controls. Genotyping for GAS5 and CASC8 polymorphisms was performed using real-time PCR, and the association with CRC risk was evaluated using logistic regression to calculate odds ratios (OR) and 95% confidence intervals (CI). The carriers of GAS5 rs145204276 del allele was significantly associated with increased CRC risk (OR: 2.13, 95% CI: 1.24-3.63, = 0.005) in a dominant model. In the subgroup analysis, the association of GAS5 rs145204276 ins/del polymorphism was restricted to distal colon cancer cases (OR: 2.98, 95% CI: 1.57-5.66, = 0.001), advanced tumor stages (III + IV) (OR: 2.54, 95% CI: 1.31-4.91, = 0.007), and poorly differentiated tumors (G3) (OR: 3.98, 95% CI: 1.49-10.59, = 0.009). No significant correlation was found for the CASC8 rs10505477 A>G polymorphism. GAS5 rs145204276 polymorphism may influence CRC susceptibility, particularly in distal tumors and advanced stages. However, CASC8 rs10505477 polymorphism showed no association with CRC risk in this Romanian cohort.
结直肠癌（CRC）是癌症相关发病和死亡的主要原因之一，受到遗传和表观遗传因素的影响。长链非编码RNA（lncRNA）如GAS5和CASC8与癌症易感性有关。本研究旨在评估罗马尼亚人群中GAS5 rs145204276插入/缺失和CASC8 rs10505477 A>G多态性与CRC风险的关联。进行了一项病例对照研究，包括156例CRC患者和195例健康对照。使用实时PCR对GAS5和CASC8多态性进行基因分型，并使用逻辑回归计算比值比（OR）和95%置信区间（CI）来评估与CRC风险的关联。在显性模型中，GAS5 rs145204276缺失等位基因的携带者与CRC风险增加显著相关（OR：2.13，95% CI：1.24 - 3.63，P = 0.005）。在亚组分析中，GAS5 rs145204276插入/缺失多态性的关联仅限于远端结肠癌病例（OR：2.98，95% CI：1.57 - 5.66，P = 0.001）、晚期肿瘤阶段（III + IV）（OR：2.54，95% CI：1.31 - 4.91，P = 0.007）和低分化肿瘤（G3）（OR：3.98，95% CI：1.49 - 10.59，P = 0.009）。未发现CASC8 rs10505477 A>G多态性有显著相关性。GAS5 rs145204276多态性可能影响CRC易感性，特别是在远端肿瘤和晚期阶段。然而，在这个罗马尼亚队列中，CASC8 rs10505477多态性与CRC风险无关。
