Fahad Shah, Khan Faheem Ahmed, Pandupuspitasari Nuruliarizki Shinta, Ahmed Muhammad Mahmood, Liao Yu Cai, Waheed Muhammad Tahir, Sameeullah Muhammad, Hussain Saddam, Saud Shah, Hassan Shah, Jan Amanullah, Jan Mohammad Tariq, Wu Chao, Chun Ma Xiao, Huang Jianliang
National Key Laboratory of Crop Genetic Improvement, MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, 430070, Hubei, China.
Biotechnol Lett. 2015 Feb;37(2):265-79. doi: 10.1007/s10529-014-1699-7. Epub 2014 Oct 18.
Infectious diseases and cancers are some of the commonest causes of deaths throughout the world. The previous two decades have witnessed a combined endeavor across various biological sciences to address this issue in novel ways. The advent of recombinant DNA technologies has provided the tools for producing recombinant proteins that can be used as therapeutic agents. A number of expression systems have been developed for the production of pharmaceutical products. Recently, advances have been made using plants as bioreactors to produce therapeutic proteins directed against infectious diseases and cancers. This review highlights the recent progress in therapeutic protein expression in plants (stable and transient), the factors affecting heterologous protein expression, vector systems and recent developments in existing technologies and steps towards the industrial production of plant-made vaccines, antibodies, and biopharmaceuticals.
传染病和癌症是全球最常见的一些死因。在过去二十年中，各个生物科学领域共同努力，以新颖的方式解决这一问题。重组DNA技术的出现为生产可用作治疗剂的重组蛋白提供了工具。已经开发了多种表达系统用于生产药品。最近，利用植物作为生物反应器来生产针对传染病和癌症的治疗性蛋白取得了进展。本综述重点介绍了植物中治疗性蛋白表达（稳定和瞬时）的最新进展、影响异源蛋白表达的因素、载体系统以及现有技术的最新发展，以及植物制造的疫苗、抗体和生物制药走向工业化生产的步骤。
