Espínola Emilio E, Amarilla Alberto A, Martínez Magaly, Aquino Víctor H, Russomando Graciela
Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Paraguay.
Departamento de Biología Molecular y Genética, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Paraguay ; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Open Virol J. 2014 Sep 29;8:9-13. doi: 10.2174/1874357901408010009. eCollection 2014.
Influenza virus is associated with upper respiratory tract infections. The fourth influenza pandemic was declared in 2009. The aim of this study was to determine the genetic variability of the 2009 H1N1 pandemic virus circulating in Paraguay. Nasal swabs were collected from 181 patients with flu symptoms managed at the Hospital of the Medical School in Asunción, Paraguay, between August and October 2009. Virus detection was carried out by real-time reverse transcription-polymerase chain reaction, followed by sequencing of the hemagglutinin and neuraminidase genes, and phylogenetic analysis. H1N1pdm09 was detected in 14.9% (27/181) of the suspected cases. Analysis of 13 samples showed that these viruses the clustered in a single genetic group. Neither the mutation related to exacerbation of disease (D239G in hemagglutinin) nor that related to antiviral resistance (H275Y in neuraminidase), both detected in neighboring countries, were found. This genetic analysis of H1N1pdm09 will help to understand the spread of the disease.
流感病毒与上呼吸道感染有关。2009年宣布了第四次流感大流行。本研究的目的是确定在巴拉圭传播的2009年H1N1大流行病毒的基因变异性。2009年8月至10月期间，从巴拉圭亚松森医学院医院治疗的181名有流感症状的患者中采集了鼻拭子。通过实时逆转录-聚合酶链反应进行病毒检测，随后对血凝素和神经氨酸酶基因进行测序，并进行系统发育分析。在14.9%（27/181）的疑似病例中检测到H1N1pdm09。对13个样本的分析表明，这些病毒聚集在一个单一的基因群中。在邻国检测到的与疾病加重相关的突变（血凝素中的D239G）和与抗病毒耐药性相关的突变（神经氨酸酶中的H275Y）均未发现。对H1N1pdm09的这种基因分析将有助于了解该疾病的传播情况。
