Casazza Krista, Hanks Lynae J, Fields David A
Department of Nutrition Sciences, University of Alabama at Birmingham , Birmingham, AL, USA.
Division of Nephrology, School of Medicine, University of Alabama at Birmingham , Birmingham, AL, USA.
Bonekey Rep. 2014 Oct 8;3:577. doi: 10.1038/bonekey.2014.72. eCollection 2014.
Human breast milk (HBM) contains numerous bioactive components, recently shown to be associated with growth and body composition in breastfed offspring. Reciprocity in adipogenic and osteogenic pathways suggests bone mass may also be influenced by these components. The association between bioactive components found in HBM and bone mineral content (BMC), to our knowledge, is unknown. The purpose of this proof-of-principle study was to evaluate the association between specific bioactive components in HBM in exclusively breastfed infants and skeletal health in the first 6 months of life and examine potential gender differences in these associations. Thirty-five mother-infant dyads were followed from 1 to 6 months. The contents of a single breast expression were used for analyses of bioactive components (insulin, glucose, leptin, interleukin-6 and tumor necrosis factor-α (TNFα), whereas BMC was evaluated by dual-energy X-ray absorptiometry. In the total sample, there was a positive association between TNFα and BMC at 1 (P=0.004) and 6 months (P=0.007). When stratified by sex, females exhibited a positive association between BMC and glucose and an inverse relationship between BMC and TNF-α at 1 month with TNF-α strengthening (P=0.006) at 6 months. In males, at 6 months a positive relationship between BMC and HBM glucose and an inverse relationship with HBM leptin were observed with no associations observed at 1 month. Although preliminary, the associations between bioactive components in HBM highlight the importance HBM has on bone accretion. It is critically important to identify factors in HBM that contribute to optimal bone health.
人乳（HBM）含有众多生物活性成分，最近研究表明这些成分与母乳喂养后代的生长及身体组成有关。脂肪生成和成骨途径的相互作用表明，这些成分可能也会影响骨量。据我们所知，HBM中发现的生物活性成分与骨矿物质含量（BMC）之间的关联尚不清楚。这项原理验证研究的目的是评估纯母乳喂养婴儿HBM中特定生物活性成分与出生后头6个月骨骼健康之间的关联，并研究这些关联中潜在的性别差异。35对母婴从1个月随访至6个月。单次挤奶的乳汁用于分析生物活性成分（胰岛素、葡萄糖、瘦素、白细胞介素-6和肿瘤坏死因子-α（TNFα）），而BMC通过双能X线吸收法进行评估。在总样本中，TNFα与1个月时的BMC（P=0.004）及6个月时的BMC（P=0.007）呈正相关。按性别分层时，女性在1个月时BMC与葡萄糖呈正相关，BMC与TNF-α呈负相关，且在6个月时TNF-α相关性增强（P=0.006）。在男性中，6个月时观察到BMC与HBM葡萄糖呈正相关，与HBM瘦素呈负相关，1个月时未观察到相关性。尽管是初步研究，但HBM中生物活性成分之间的关联突出了HBM对骨量增加的重要性。确定HBM中有助于实现最佳骨骼健康的因素至关重要。
