Marks Haley L, Pishko Michael V, Jackson George W, Coté Gerard L
Department of Biomedical Engineering, Texas A&M University , College Station, Texas 77843, United States.
Anal Chem. 2014 Dec 2;86(23):11614-9. doi: 10.1021/ac502541v. Epub 2014 Nov 10.
Surface-enhanced Raman scattering (SERS) optical nanoprobes offer a number of advantages for ultrasensitive analyte detection. These functionalized colloidal nanoparticles are a multifunctional assay component. providing a platform for conjugation to spectral tags, stabilizing polymers, and biorecognition elements such as aptamers or antibodies. We demonstrate the design and characterization of a SERS-active nanoprobe and investigate the nanoparticles' biorecognition capabilities for use in a competitive binding assay. Specifically, the nanoprobe is designed for the quantification of bisphenol A (BPA) levels in the blood after human exposure to the toxin in food and beverage plastic packaging. The nanoprobes demonstrated specific affinity to a BPA aptamer with a dissociation constant Kd of 54 nM, and provided a dose-dependent SERS spectra with a limit of detection of 3 nM. Our conjugation approach shows the versatility of colloidal nanoparticles in assay development, acting as detectable spectral tagging elements and biologically active ligands concurrently.
表面增强拉曼散射（SERS）光学纳米探针为超灵敏分析物检测提供了诸多优势。这些功能化的胶体纳米颗粒是一种多功能检测组件，为与光谱标签、稳定聚合物以及适配体或抗体等生物识别元件的结合提供了一个平台。我们展示了一种具有SERS活性的纳米探针的设计与表征，并研究了该纳米颗粒在竞争性结合检测中用于生物识别的能力。具体而言，该纳米探针设计用于定量检测人体因接触食品和饮料塑料包装中的毒素后血液中的双酚A（BPA）水平。这些纳米探针表现出对BPA适配体的特异性亲和力，解离常数Kd为54 nM，并提供了剂量依赖性的SERS光谱，检测限为3 nM。我们的缀合方法展示了胶体纳米颗粒在检测开发中的多功能性，它们同时充当可检测的光谱标记元件和生物活性配体。
