粒子微束照射后用单细胞生物传感器测量代谢氧消耗
Metabolic oxygen consumption measurement with a single-cell biosensor after particle microbeam irradiation.
作者信息
Xu Yanping, Zhang Bo, Messerli Mark, Randers-Pehrson Gerhard, Hei Tom K, Brenner David J
机构信息
Radiological Research Accelerator Facility (RARAF), Center for Radiological Research, Columbia University, 136 S. Broadway, Irvington, NY, 10533, USA.
Center for Radiological Research, Columbia University, New York, NY, 10032, USA.
出版信息
Radiat Environ Biophys. 2015 Mar;54(1):137-144. doi: 10.1007/s00411-014-0574-1. Epub 2014 Oct 22.
A noninvasive, self-referencing biosensor/probe system has been integrated into the Columbia University Radiological Research Accelerator Facility Microbeam II end station. A single-cell oxygen consumption measurement has been conducted with this type of oxygen probe in 37° C Krebs-Ringer Bicarbonate buffer immediately before and after a single-cell microbeam irradiation. It is the first such measurement made for a microbeam irradiation, and a six fold increment of oxygen flux induced during a 15-s period of time has been observed following radiation exposure. The experimental procedure and the results are discussed.
一种非侵入式、自参考生物传感器/探针系统已集成到哥伦比亚大学放射研究加速器设施微束II终端站。在37°C的 Krebs-Ringer 碳酸氢盐缓冲液中，使用这种类型的氧探针在单细胞微束照射前后立即进行了单细胞耗氧量测量。这是首次针对微束照射进行此类测量，并且在辐射暴露后的15秒内观察到氧通量增加了六倍。本文讨论了实验过程和结果。
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