Drexler Guido A, Siebenwirth Christian, Drexler Sophie E, Girst Stefanie, Greubel Christoph, Dollinger Günther, Friedl Anna A
Department of Radiation Oncology, Ludwig-Maximilians-Universität München, Munich, Germany.
Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
Radiat Oncol. 2015 Feb 18;10:42. doi: 10.1186/s13014-015-0350-7.
Ion microbeams are important tools in radiobiological research. Still, the worldwide number of ion microbeam facilities where biological experiments can be performed is limited. Even fewer facilities combine ion microirradiation with live-cell imaging to allow microscopic observation of cellular response reactions starting very fast after irradiation and continuing for many hours. At SNAKE, the ion microbeam facility at the Munich 14 MV tandem accelerator, a large variety of biological experiments are performed on a regular basis. Here, recent developments and ongoing research projects at the ion microbeam SNAKE are presented with specific emphasis on live-cell imaging experiments. An overview of the technical details of the setup is given, including examples of suitable biological samples. By ion beam focusing to submicrometer beam spot size and single ion detection it is possible to target subcellular structures with defined numbers of ions. Focusing of high numbers of ions to single spots allows studying the influence of high local damage density on recruitment of damage response proteins.
离子微束是放射生物学研究中的重要工具。然而，全球范围内能够进行生物实验的离子微束设施数量有限。将离子微辐照与活细胞成像相结合，以便在辐照后非常迅速地开始并持续数小时对细胞反应进行微观观察的设施更是少之又少。在慕尼黑14兆伏串联加速器的离子微束设施SNAKE，定期开展各种各样的生物实验。本文介绍了离子微束SNAKE的最新进展和正在进行的研究项目，特别着重于活细胞成像实验。文中给出了该装置技术细节的概述，包括合适生物样品的实例。通过将离子束聚焦到亚微米束斑尺寸并进行单离子检测，可以用确定数量的离子靶向亚细胞结构。将大量离子聚焦到单个点上能够研究高局部损伤密度对损伤反应蛋白募集的影响。
