Lin Li, Liu Yaqiong, Zhao Weihua, Sun Bo, Chen Qi
Department of Obstetrics and Gynecology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen 518000, China.
Department of Obstetrics and Gynecology, Guangzhou Women and Children's Medical Center Guangzhou 510120, China.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6072-8. eCollection 2014.
To identify the clinical significance of Wnt5A expression in the development and progression of cervical cancer.
Real-time PCR was performed in 8 pairs of surgically resected cervical cancer and adjacent normal cervical tissues. Immunohistochemistry was performed to examine Wnt5A expression in 94 paraffin-embedded cervical cancer samples. Associations of Wnt5A expression with clinicopathological factors and clinical survival were analyzed.
Wnt5A expression was overexpressed in cervical cancer tissues compared with adjacent normal cervix. Wnt5A expression tended to be positively correlated with lymph nodes metastasis (P = 0.028) and recurrence (P = 0.009). Moreover, patients with higher Wnt5A expression in cancer tissues had better overall (P = 0.004) and recurrent-free survival (P = 0.012) than those with lower Wnt5A expression. Multivariate analysis revealed that Wnt5A was an independent prognostic factor (P = 0.026) for predicting overall survival of cervical cancer patients.
Upregulation of Wnt5A was associated with metastasis and progression of cervical cancer. The results of our study unravel the significance of Wnt/Ca2+ signaling in cervical cancer.
确定Wnt5A表达在宫颈癌发生发展中的临床意义。
对8对手术切除的宫颈癌组织及相邻正常宫颈组织进行实时聚合酶链反应。对94例石蜡包埋的宫颈癌样本进行免疫组织化学检测，以检测Wnt5A的表达。分析Wnt5A表达与临床病理因素及临床生存的相关性。
与相邻正常宫颈相比，Wnt5A在宫颈癌组织中过表达。Wnt5A表达倾向于与淋巴结转移（P = 0.028）和复发（P = 0.009）呈正相关。此外，癌组织中Wnt5A表达较高的患者比Wnt5A表达较低的患者具有更好的总生存期（P = 0.004）和无复发生存期（P = 0.012）。多因素分析显示，Wnt5A是预测宫颈癌患者总生存期的独立预后因素（P = 0.026）。
Wnt5A的上调与宫颈癌的转移和进展相关。我们的研究结果揭示了Wnt/Ca2+信号通路在宫颈癌中的意义。
