Lee Hye Won, Kim Hyojun, Ryuk Jin Ah, Kil Ki-Jung, Ko Byoung Seob
Korean Medicine Based Herbal Drug Development Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine Republic of Korea.
College of Oriental Medicine, Joongbu University Republic of Korea.
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6179-85. eCollection 2014.
Samul-tang (Si-Wu-Tang, SMT), a kind of herbal medicines, has been used for the hemato-deficient disease for hundreds of years. In this work, investigate the anti-anemia activity of the H2O extracts from constituent herbal medicines of Samul-tang in an anemia model induced by intravenous infection of phenylhydrazine-HCL (PHZ) at 10 mg/kg for 4 days. After PHZ injection, female Sparague-Dawley rats were administrated extracts from constituent herbal medicines of SMT (300 mg/kg/day, p.o.) daily for 1 week. Results showed that sever hemolysis was induced by PHZ. For Paeonia lactiflora (PL2) H2O extract treated groups, the concentration of hemoglobin, hematocrit and red blood cells number increased much more significantly than PHZ-treated group. Moreover, Angelica gigas (AG), Angelica. acutiloba (AA), Paeonia lactiflora (PL2) and Rehmannia glutinosa (RG) extract administration significantly improved serum erythropoietin concentration. The activity of aminolevulinic acid dehydrates (ALDL) in liver homegenate was increased in Angelica gigas(AA), Paeonia lactiflora (PL2) and Rehmannia glutinosa (RG) treated group.
四物汤是一种草药，数百年来一直用于治疗血虚症。在本研究中，以10mg/kg的盐酸苯肼（PHZ）静脉注射4天诱导贫血模型，研究四物汤组成草药的水提取物的抗贫血活性。注射PHZ后，对雌性斯普拉格-道利大鼠每天灌胃给予四物汤组成草药的提取物（300mg/kg/天，口服），持续1周。结果显示，PHZ诱导了严重溶血。对于芍药（PL2）水提取物处理组，血红蛋白浓度、血细胞比容和红细胞数量的增加比PHZ处理组显著得多。此外，当归（AG）、东当归（AA）、芍药（PL2）和熟地黄（RG）提取物给药显著提高了血清促红细胞生成素浓度。当归（AA）、芍药（PL2）和熟地黄（RG）处理组肝脏匀浆中氨基乙酰丙酸脱水酶（ALDL）的活性增加。
