Liofilchem（®）彩色耐万古霉素肠球菌和万古霉素MIC测试条在一株vanB型新生儿粪肠球菌分离株中检测到糖肽类耐药性，该分离株对万古霉素表现出交替的敏感性和耐药性，与生物梅里埃Vitek2检测结果一致。
Liofilchem(®) Chromatic VRE and vancomycin MIC Test Strip detected glycopeptide resistance in a vanB neonatal Enterococcus faecium isolate showing alternate vancomycin susceptibility and resistance with bioMérieux Vitek2.
作者信息
Savini Vincenzo, Marrollo Roberta, Coclite Eleonora, Fusilli Paola, D'Incecco Carmine, Fazii Paolo, Gherardi Giovanni
机构信息
Clinical Microbiology and Virology, Spirito Santo Hospital Pescara (Pe), Italy.
Neonatal Intensive Care Unit and Neonatal Pathology, Spirito Santo Hospital Pescara (PE), Italy.
出版信息
Int J Clin Exp Pathol. 2014 Aug 15;7(9):6274-7. eCollection 2014.
A 1-month old neonate urine sample yielded vanB Enterococcus faecium; nevertheless, the isolate alternatively showed susceptibility and resistance to vancomycin with bioMérieux Vitek2 (cards AST592, AST632, AST586), while glycopeptide resistance was detected by Liofilchem(®) vancomycin MIC Test Strip and disc along with the Chromatic VRE chromogenic medium. This communication emphasizes that, as vanB gene may be heterogeneously expressed within a given Enterococcus population, glycopeptide resistance may be missed when using automated systems for antibiotic susceptibility testing. We suggest therefore that vancomycin in vitro activity be studied on all clinical isolates through agar methods, including use of chromogenic media.
一份1个月大新生儿的尿液样本培养出了携带vanB基因的屎肠球菌；然而，使用生物梅里埃Vitek2（卡片AST592、AST632、AST586）检测时，该分离株对万古霉素时而显示敏感，时而显示耐药，而使用Liofilchem（®）万古霉素MIC测试条和纸片以及显色VRE显色培养基检测到了糖肽类耐药。本报告强调，由于vanB基因可能在给定的肠球菌群体中异质性表达，使用自动化系统进行抗生素敏感性测试时可能会漏检糖肽类耐药。因此，我们建议通过琼脂方法，包括使用显色培养基，对所有临床分离株进行万古霉素体外活性研究。
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