Liu L, Zhang Q, Feng J, Deng L, Zeng N, Yang A, Zhang W
Research Unit of Genotoxicology, School of Public Health, Sun Yat sen Univ. of Medical Sciences, Guangzhou, China.
Mutat Res. 1996 Oct 1;370(3-4):145-50. doi: 10.1016/s0165-1218(96)00048-1.
Peripheral blood lymphocyte 8-hydroxy-2-deoxyguanosine (8-OHdG), were detected in 87 benzene-exposed and 30 control subjects by high performance liquid chromatograph coupled with an electrochemical detector system (HPLC-EC). The air concentration of benzene and its homologes in the workplace, urinary trans,trans-muconic acid (TTMA) as an internal dose of benzene exposure, were examined. The lymphocyte micronuclei (MN) as genotoxic and white blood cell (WBC) count as well as the myelotoxic markers of benzene were examined. Exposure to low, medium and high concentrations of benzene resulted in increased levels of 8-OHdG, which were 4.67, 26.12 and 29.89/10(5) dG, respectively, However, the 8-OHdG level observed in the control group was 3.738/10(5) dG). A good correlation between 8-OHdG formation and the groups exposed to external and internal benzene was observed (r = 0.77, 0.64, respectively). There was also a correlation between 8-OHdG and MN formation (r = 0.50). WBC levels were within normal range in all benzene-exposed subjects. It may be concluded that: benzene induced DNA oxidative damage in occupational exposure workers. The major factors influencing blood the 8-OHdG level were sex and toluene.
采用高效液相色谱-电化学检测系统（HPLC-EC），对87名苯接触者和30名对照者外周血淋巴细胞8-羟基-2'-脱氧鸟苷（8-OHdG）进行检测。同时检测工作场所空气中苯及其同系物的浓度，以及作为苯内暴露剂量的尿反，反式粘康酸（TTMA）。检测淋巴细胞微核（MN）作为遗传毒性指标，以及白细胞（WBC）计数作为苯的骨髓毒性指标。低、中、高浓度苯暴露均导致8-OHdG水平升高，分别为4.67、26.12和29.89/10⁵dG，而对照组的8-OHdG水平为3.738/10⁵dG。观察到8-OHdG形成与外部和内部苯暴露组之间存在良好的相关性（分别为r = 0.77、0.64）。8-OHdG与MN形成之间也存在相关性（r = 0.50）。所有苯接触者的白细胞水平均在正常范围内。可以得出结论：苯可导致职业暴露工人DNA氧化损伤。影响血液中8-OHdG水平的主要因素是性别和甲苯。
