Li Jian, Chen Jiangtao, Xie Dongde, Monte-Nguba Santiago-M, Eyi Juan Urbano Monsuy, Matesa Rocio Apicante, Obono Maximo Miko Ondo, Ehapo Carlos Sala, Yang Liye, Lu Danjie, Yang Hui, Yang Hui-Tian, Lin Min
Pathog Glob Health. 2014 Oct;108(7):339-43. doi: 10.1179/2047773214Y.0000000158. Epub 2014 Oct 27.
Drug resistance against Plasmodium falciparum has been recognized as the crucial obstacle to curbing mortality and morbidity from malaria. To investigate the distribution and pattern of multidrug resistance 1 (pfmdr1) gene polymorphisms in P. falciparum, isolates collected from the malaria high-endemic Bioko Island, Equatorial Guinea.
Blood samples were collected from 217 patients with P. falciparum malaria during rainy season in 2012 on Bioko Island. These samples were extracted using Chelex to obtain parasite DNA. Nest-polymerase chain reaction (PCR) and sequencing were employed to detect mutations (N86Y, E130K, Y184F, S1034C, N1042D, V1109I, and D1246Y) and haplotypes in pfmdr1 gene.
A total of 151 samples were successfully detected for pfmdr1 mutations from the 217 patients. Pfmdr1 mutations were found in 91·39% (138/151) P. falciparum isolates. However, no mutation at 130 and 1109 was identified from these samples. Four haplotypes coding 86, 184, 1034, 1,042, and 1,246 were found including NYSND, YYSND, NFSND, and YFSND, which accounted for 8·61% (13/151), 2·65% (4/151), 29·80% (45/151), and 58·94% (89/151), respectively.
Our results exhibited hypersensitivity to lumefantrine (LU) and mefloquine (MQ) and resistance to chloroquine (CQ) and amodiaquine (AQ) in P. falciparum isolates from Bioko Island. This information will be useful for anti-malarial drug policy in Equatorial Guinea.
恶性疟原虫耐药性已被视为遏制疟疾死亡率和发病率的关键障碍。为调查赤道几内亚疟疾高流行的比奥科岛恶性疟原虫多药耐药1（pfmdr1）基因多态性的分布及模式，收集了疟原虫分离株。
2012年雨季期间，在比奥科岛从217例恶性疟患者采集血样。使用螯合树脂提取这些样本以获得疟原虫DNA。采用巢式聚合酶链反应（PCR）和测序检测pfmdr1基因中的突变（N86Y、E130K、Y184F、S1034C、N1042D、V1109I和D1246Y）及单倍型。
在217例患者中，共成功检测到151份样本存在pfmdr1突变。在91.39%（138/151）的恶性疟原虫分离株中发现了pfmdr1突变。然而，在这些样本中未鉴定出130和1109位点的突变。发现了编码86、184、1034、1042和1246位点的四种单倍型，分别为NYSND、YYSND、NFSND和YFSND，占比分别为8.61%（13/151）、2.65%（4/151）、29.80%（45/151）和58.94%（89/151）。
我们的结果显示，比奥科岛恶性疟原虫分离株对本芴醇（LU）和甲氟喹（MQ）敏感，对氯喹（CQ）和阿莫地喹（AQ）耐药。该信息将有助于赤道几内亚制定抗疟药物政策。
