Prakash Indra, Bunders Cynthia, Devkota Krishna P, Charan Romila D, Ramirez Catherine, Snyder Tara M, Priedemann Christopher, Markosyan Avetik, Jarrin Cyrille, Halle Robert Ter
The Coca-Cola Company, Atlanta, GA 30313, USA.
AMRI-Albany, Analytical Development, Albany, NY 12212, USA.
Molecules. 2014 Oct 28;19(11):17345-55. doi: 10.3390/molecules191117345.
To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.
为满足对天然高效甜味剂不断增长的需求，以减少食品和饮料中的热量，我们将目光投向了甜菊糖苷。在这项工作中，我们报道了使用葡萄糖基转移酶将莱鲍迪苷A生物转化为莱鲍迪苷I的过程。这种生物转化反应以1→3键添加了一个糖单元。我们利用一维和二维核磁共振光谱（1H、13C、COSY、HSQC-DEPT、HMBC、一维TOCSY和NOESY）以及质谱数据对莱鲍迪苷I进行了全面表征。
