Yun Jieun, Kim Bo Geun, Kang Jong Soon, Park Song-Kyu, Lee Kiho, Hyun Dong-Hoon, Kim Hwan Mook, In Man-Jin, Kim Dong Chung
1 Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology , Ochang, Republic of Korea.
J Med Food. 2015 Jan;18(1):102-8. doi: 10.1089/jmf.2013.3138.
This study was performed to elucidate the effect of a lipid-soluble ginseng extract (LSGE) on cancer invasion and metastasis. The LSGE, even at noncytotoxic concentrations, potently inhibited invasion and migration of B16F10 mouse melanoma cells in a dose-dependent manner. In the presence of 3 μg/mL of LSGE, the invasion and migration of B16F10 cells were significantly inhibited by 98.1% and 71.4%, respectively. Furthermore, the LSGE decreased mRNA and protein levels of matrix metalloproteinase (MMP)-2 in B16F10 cells, leading to a decrease in MMP-2 activity. After B16F10 cells were intravenously injected in the tail vein of C57BL/6 mice, 1000 mg/kg/day of LSGE was orally administered for 13 days, after which lung metastasis of cancer cells was inhibited by 59.3%. These findings indicate that LSGE inhibits cancer cell invasion and migration in vitro and lung metastasis of melanoma cells in vivo by inhibiting MMP-2 expression.
本研究旨在阐明脂溶性人参提取物（LSGE）对癌症侵袭和转移的影响。即使在无细胞毒性浓度下，LSGE也能以剂量依赖的方式有效抑制B16F10小鼠黑色素瘤细胞的侵袭和迁移。在存在3μg/mL LSGE的情况下，B16F10细胞的侵袭和迁移分别被显著抑制了98.1%和71.4%。此外，LSGE降低了B16F10细胞中基质金属蛋白酶（MMP）-2的mRNA和蛋白质水平，导致MMP-2活性降低。在将B16F10细胞静脉注射到C57BL/6小鼠的尾静脉后，每天口服1000mg/kg的LSGE，持续13天，之后癌细胞的肺转移被抑制了59.3%。这些发现表明，LSGE通过抑制MMP-2表达在体外抑制癌细胞侵袭和迁移，并在体内抑制黑色素瘤细胞的肺转移。
