翻译起始位点下游的序列调控矮牵牛两个rbcS基因的定量表达。
Sequences downstream of translation start regulate quantitative expression of two petunia rbcS genes.
作者信息
Dean C, Favreau M, Bond-Nutter D, Bedbrook J, Dunsmuir P
机构信息
Advanced Genetic Sciences, Oakland, California 94608.
出版信息
Plant Cell. 1989 Feb;1(2):201-8. doi: 10.1105/tpc.1.2.201.
Abstract
We investigated the basis for quantitative differences in leaf expression of the petunia genes (rbcS) encoding the small subunit of ribulose bisphosphate carboxylase. The most abundantly, SSU301, and the most weakly, SSU911, expressed petunia rbcS genes maintained their differential expression when transferred to tobacco, indicating that the determinants of quantitative expression are intrinsic to these rbcS genes. Analysis of chimeric genes in which the sequences of SSU301 and SSU911 had been exchanged at the translation start showed that sequences both 5' and 3' to the start codon contribute to differences in steady-state mRNA levels. The sequences 3' to the translation initiation codon were investigated by preparing chimeric genes in which sequences of the SSU301 and SSU911 were exchanged between each intron and at the translation termination codon. The results showed that sequences downstream of the coding region contribute to quantitative differences in expression of SSU301 and SSU911, and nuclear run-on transcription experiments indicated that the 3' sequences affect transcription rates of the rbcS genes.
摘要
我们研究了矮牵牛中编码核酮糖二磷酸羧化酶小亚基的基因（rbcS）在叶片中表达量存在定量差异的原因。表达量最高的SSU301和表达量最低的SSU911这两个矮牵牛rbcS基因在转入烟草后仍保持其差异表达，这表明定量表达的决定因素是这些rbcS基因所固有的。对在翻译起始位点处交换了SSU301和SSU911序列的嵌合基因进行分析表明，起始密码子5'端和3'端的序列均对稳态mRNA水平的差异有影响。通过制备在每个内含子之间以及翻译终止密码子处交换了SSU301和SSU911序列的嵌合基因，对翻译起始密码子3'端的序列进行了研究。结果表明，编码区下游的序列对SSU301和SSU911表达量的定量差异有影响，并且细胞核连续转录实验表明3'端序列影响rbcS基因的转录速率。
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本文引用的文献
1
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Plant Physiol. 1980 Feb;65(2):305-8. doi: 10.1104/pp.65.2.305.
2
Wound-inducible expression of a potato inhibitor II-chloramphenicol acetyltransferase gene fusion in transgenic tobacco plants.马铃薯蛋白酶抑制剂 II-氯霉素乙酰转移酶基因融合在转基因烟草植株中的诱导型表达。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):744-8. doi: 10.1073/pnas.84.3.744.
3
Transcriptional and post-transcriptional regulation of soybean seed protein mRNA levels.大豆种子蛋白 mRNA 水平的转录后调控。
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2123-7. doi: 10.1073/pnas.83.7.2123.
4
Linkage and homology analysis divides the eight genes for the small subunit of petunia ribulose 1,5-bisphosphate carboxylase into three gene families.连锁和同源分析将矮牵牛花核酮糖 1,5-二磷酸羧化酶小亚基的 8 个基因划分为三个基因家族。
Proc Natl Acad Sci U S A. 1985 Aug;82(15):4964-8. doi: 10.1073/pnas.82.15.4964.
5
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6
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7
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8
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9
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10
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