Nair Praveen, Malhotra Anshoo, Dhawan Devinder K
Department of Biomechanics, Sports Authority of India, Bangalore, 560056, India.
Mol Cell Biochem. 2015 Feb;400(1-2):51-6. doi: 10.1007/s11010-014-2261-6. Epub 2014 Oct 31.
The aim of the present study was to assess the cumulative effects of curcumin and quercetin in inducing apoptosis during benzo(a)pyrene (BP) (100 mg/Kg body weight)-induced lung carcinogenesis in mice. BP treatment resulted in a significant increase in the protein expression of Bcl-2 whereas expression of Bax was significantly decreased. Further, BP treatment brought about a significant decrease in the activities of caspase 3, caspase 9 as well as the number of apoptotic cells. Interestingly, separate as well as combined supplementation of curcumin (60 mg/kg body weight) and quercetin (40 mg/kg body weight) to BP-treated animals resulted in a significant decrease in the protein expression of Bcl-2 but caused a significant increase in the protein expression of Bax along with a noticeable improvement in the number of apoptotic cells. Also, supplementation with curcumin and quercetin separately to BP-treated mice brought a significant improvement in the enzyme activities of caspase 9 as well as caspase 3 but the improvement was more pronounced following combined treatment. Therefore, curcumin and quercetin, if given in combination shall exhibit enhanced chemopreventive potential against development of lung carcinogenesis by stimulating the apoptotic machinery.
本研究的目的是评估姜黄素和槲皮素在苯并(a)芘（BP）（100毫克/千克体重）诱导的小鼠肺癌发生过程中诱导细胞凋亡的累积效应。BP处理导致Bcl-2蛋白表达显著增加，而Bax表达显著降低。此外，BP处理使半胱天冬酶3、半胱天冬酶9的活性以及凋亡细胞数量显著减少。有趣的是，单独或联合给予BP处理动物姜黄素（60毫克/千克体重）和槲皮素（40毫克/千克体重），导致Bcl-2蛋白表达显著降低，但Bax蛋白表达显著增加，同时凋亡细胞数量有明显改善。此外，单独给予BP处理小鼠姜黄素和槲皮素可使半胱天冬酶9以及半胱天冬酶3的酶活性有显著改善，但联合处理后的改善更明显。因此，姜黄素和槲皮素联合使用时，通过刺激凋亡机制，对肺癌发生的化学预防潜力将增强。
