de Turenne-Tessier M, Ooka T, Calender A, de Thé G, Daillie J
Laboratoire d'Epidémiologie et Immunovirologie des Tumeurs et de Virologie Moléculaire, Faculté de Médecine Alexis Carrel, Lyon, France.
Int J Cancer. 1989 Jan 15;43(1):45-8. doi: 10.1002/ijc.2910430111.
Epstein-Barr virus (EBV) has long been implicated in nasopharyngeal carcinoma (NPC). Recent studies in our and other laboratories have shown a correlation between the disease and high antibody titers to EBV-specific DNase. These data led us to also examine serial sera from healthy adults and patients with infectious mononucleosis or NPC, for their capacity to neutralize the EBV-specific thymidine kinase (TK) activity from chemically induced EBV-carrying human lymphoblastoid cells. Our results were the following: (i) sera were found that efficiently blocked the EBV-specific TK activity of induced-Raji TK- cell extracts, but not the host-cell TK activity from EBV-negative BJAB cells; (ii) a relationship appeared between high levels of EBV-specific TK-neutralizing activity in sera and NPC pathology, even though in this preliminary study the degrees of EBV-induced TK-blocking activity detected in sera were not significantly correlated with EBV-specific antibody titers; (iii) the EBV-induced TK-neutralizing activity was found in the main IgG fraction derived from NPC sera. These data must be compared with other known antibody responses to EBV for their clinical interest in NPC control.
长期以来，人们一直认为爱泼斯坦-巴尔病毒（EBV）与鼻咽癌（NPC）有关。我们实验室和其他实验室最近的研究表明，该疾病与针对EBV特异性脱氧核糖核酸酶的高抗体滴度之间存在相关性。这些数据促使我们检测健康成年人、传染性单核细胞增多症患者或鼻咽癌患者的系列血清，以评估它们中和化学诱导的携带EBV的人淋巴母细胞样细胞中EBV特异性胸苷激酶（TK）活性的能力。我们的结果如下：（i）发现血清能有效阻断诱导的拉吉TK-细胞提取物的EBV特异性TK活性，但不能阻断EBV阴性BJAB细胞的宿主细胞TK活性；（ii）血清中高水平的EBV特异性TK中和活性与鼻咽癌病理之间似乎存在关联，尽管在这项初步研究中，血清中检测到的EBV诱导的TK阻断活性程度与EBV特异性抗体滴度没有显著相关性；（iii）在源自鼻咽癌血清的主要IgG组分中发现了EBV诱导的TK中和活性。为了它们对鼻咽癌控制的临床意义，必须将这些数据与其他已知的针对EBV的抗体反应进行比较。
