Yi Ji, Chen Siyu, Backman Vadim, Zhang Hao F
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston 60208, USA.
Department of Biomedical Engineering, Northwestern University, 2145 Sheridan Rd., Evanston 60208, USA ; Department of Ophthalmology, Northwestern University, 2145 Sheridan Rd., Evanston 60208, USA.
Biomed Opt Express. 2014 Sep 15;5(10):3603-12. doi: 10.1364/BOE.5.003603. eCollection 2014 Oct 1.
Although hemoglobin oxygen saturation (sO2) in the microvasculature is an essential physiological parameter of local tissue functions, non-invasive measurement of microvascular sO2 is still challenging. Here, we demonstrated that visible-light optical coherence tomography (vis-OCT) can simultaneously provide three-dimensional anatomical tissue morphology, visualize microvasculature at the capillary level, and measure sO2 from the microvasculature in vivo. We utilized speckle contrast caused by the moving blood cells to enhance microvascular imaging. We applied a series of short-time inverse Fourier transforms to obtain the spectroscopic profile of blood optical attenuation, from which we quantified sO2. We validated the sO2 measurement in mouse ears in vivo through hypoxia and hyperoxia challenges. We further demonstrated that vis-OCT can continuously monitor dynamic changes of microvascular sO2.
尽管微血管中的血红蛋白氧饱和度（sO2）是局部组织功能的一个重要生理参数，但微血管sO2的无创测量仍然具有挑战性。在此，我们证明了可见光光学相干断层扫描（vis-OCT）能够同时提供三维解剖组织形态，在毛细血管水平可视化微血管，并在体内测量微血管的sO2。我们利用移动血细胞引起的散斑对比度来增强微血管成像。我们应用一系列短时逆傅里叶变换来获得血液光学衰减的光谱轮廓，从中量化sO2。我们通过缺氧和高氧挑战在体内验证了小鼠耳部的sO2测量。我们进一步证明了vis-OCT能够连续监测微血管sO2的动态变化。
