The Key Laboratory of Environment and Gene Related to Diseases, Ministry of Education, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, People's Republic of China.
Int J Legal Med. 2011 Jul;125(4):581-5. doi: 10.1007/s00414-010-0519-4. Epub 2010 Nov 3.
In the present study, we investigated 21 short tandem repeat (STR) loci (D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, D5S2500), which are not included in the Combined DNA Index System and Amelogenin locus in 104 randomly selected healthy autochthonous individuals from the Tibetan ethnic minority group residing in the Lhasa region, Tibet Autonomous Region of China. Allelic frequencies, common forensic statistical parameters, and the Hardy-Weinberg equilibrium in this population were calculated with a modified PowerState V12.xls. A total of 143 alleles were found in the Tibetan group with corresponding allelic frequencies ranging from 0.005 to 0.582. The observed heterozygosity, the expected heterozygosity, the power of discrimination, the power of exclusion, and the polymorphic information content ranged from 0.615 to 0.817, 0.559 to 0.787, 0.727 to 0.926, 0.310 to 0.632, and 0.488 to 0.760, respectively. Chi-square tests of the observed genotype frequencies and expected genotype frequencies in the samples showed no departure from the Hardy-Weinberg equilibrium at all loci except for D5S2500. Our results demonstrate that these 21 STRs are highly polymorphic and suitable for anthropological research, population genetics, and forensic paternity testing and human individual identification in this region, and can enrich Chinese ethnical genetic informational resources.
在本研究中，我们调查了 21 个短串联重复（STR）基因座（D6S474、D12ATA63、D22S1045、D10S1248、D1S1677、D11S4463、D1S1627、D3S4529、D2S441、D6S1017、D4S2408、D19S433、D17S1301、D1GATA113、D18S853、D20S482、D14S1434、D9S1122、D2S1776、D10S1435、D5S2500），这些基因座未包含在中国的联合 DNA 索引系统和 amelogenin 基因座中。我们从中国西藏自治区拉萨地区的藏族少数民族中随机选择了 104 名健康个体，计算了这些基因座的等位基因频率、常见法医统计学参数和 Hardy-Weinberg 平衡。使用改良的 PowerState V12.xls 计算了该人群中的等位基因频率、常见法医统计学参数和 Hardy-Weinberg 平衡。在藏族人群中发现了 143 个等位基因，对应的等位基因频率范围为 0.005 至 0.582。观察杂合度、期望杂合度、鉴别力、排除力和多态信息含量范围分别为 0.615 至 0.817、0.559 至 0.787、0.727 至 0.926、0.310 至 0.632 和 0.488 至 0.760。除了 D5S2500 之外，所有基因座的样本观察基因型频率和期望基因型频率的卡方检验均未偏离 Hardy-Weinberg 平衡。我们的结果表明，这 21 个 STR 基因座高度多态性，适合于该地区的人类学研究、群体遗传学、法医亲子鉴定和个体识别，并且可以丰富中国民族遗传信息资源。
