Faveri Marcelo, Figueiredo Luciene Cristina, Shibli Jamil Awad, Pérez-Chaparro Paula Juliana, Feres Magda
Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil,
Adv Exp Med Biol. 2015;830:85-96. doi: 10.1007/978-3-319-11038-7_5.
The open-ended microbial diagnostic approaches such as the complete or partial sequencing of the 16S ribosomal gene by Sanger sequencing or by pyrosequencing have provided new insights into the diversity of the oral microbiota. These techniques have recently been used to evaluate the microbiota associated with osseointegrated implants and these results have expanded the knowledge on the diversity of the microbial communities associated with peri-implantitis. Taken together, the results of these studies suggest that the diversity of the microbial community of peri-implantitis and periodontitis might not be as similar as previously thought. Although certain known periodontal pathogens may also be associated with the etiology of peri-implantitis, apparently there were many differences between these two clinical conditions, involving distinct microorganisms. Further investigations on the diversity of peri-implant microbiota would be essential in order to define effective preventive and therapeutic strategies for peri-implantitis. It is also important to standardize laboratory protocols to make the results of the open-ended diagnostic techniques based on PCR amplification more comparable throughout the different research groups.
开放式微生物诊断方法，如通过桑格测序或焦磷酸测序对16S核糖体基因进行全序列或部分测序，为口腔微生物群的多样性提供了新的见解。这些技术最近已被用于评估与骨结合种植体相关的微生物群，这些结果扩展了我们对与种植体周围炎相关的微生物群落多样性的认识。综合来看，这些研究结果表明，种植体周围炎和牙周炎的微生物群落多样性可能不像以前认为的那样相似。虽然某些已知的牙周病原体也可能与种植体周围炎的病因有关，但显然这两种临床情况之间存在许多差异，涉及不同的微生物。为了确定种植体周围炎的有效预防和治疗策略，对种植体周围微生物群的多样性进行进一步研究至关重要。标准化实验室方案也很重要，以便使基于PCR扩增的开放式诊断技术的结果在不同研究组之间更具可比性。
