Jazwa Agnieszka, Kasper Lukasz, Bak Maciej, Sobczak Mateusz, Szade Krzysztof, Jozkowicz Alicja, Sladek Krzysztof, Dulak Jozef
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387, Kraków, Poland.
Arch Immunol Ther Exp (Warsz). 2015 Apr;63(2):139-46. doi: 10.1007/s00005-014-0315-9. Epub 2014 Nov 1.
Sarcoidosis is a granulomatous disease of unknown etiology. The disease has an important inflammatory and immune component; however, its immunopathogenesis is not completely understood. Recently, the role of microRNAs (miRNAs), the small non-coding RNAs, has attracted attention as both being involved in pathogenesis and serving as disease markers. Accordingly, changes in the expression of some miRNAs have been also associated with different autoimmune pathologies. However, not much is known about the role of miRNAs in sarcoidosis. Therefore, the aim of this study was to compare the level of expression of selected miRNAs in healthy individuals and patients with sarcoidosis. We detected significantly increased level of miR-34a in peripheral blood mononuclear cells isolated from sarcoidosis patients. Moreover, significantly up-regulated levels of interferon (IFN)-γ, IFN-γ inducible protein (IP-10) and vascular endothelial growth factor were detected in sera of patients when compared to healthy subjects. Our results add to a known inflammatory component in sarcoidosis. Changes in the levels of miR-34a may suggest its involvement in the pathology of this disease.
结节病是一种病因不明的肉芽肿性疾病。该疾病具有重要的炎症和免疫成分；然而，其免疫发病机制尚未完全明确。最近，微小RNA（miRNA）这种小的非编码RNA，因其参与发病机制并作为疾病标志物而受到关注。相应地，一些miRNA表达的变化也与不同的自身免疫性疾病相关。然而，关于miRNA在结节病中的作用知之甚少。因此，本研究的目的是比较健康个体和结节病患者中所选miRNA的表达水平。我们检测到结节病患者外周血单个核细胞中miR-34a的水平显著升高。此外，与健康受试者相比，患者血清中干扰素（IFN）-γ、IFN-γ诱导蛋白（IP-10）和血管内皮生长因子的水平显著上调。我们的结果进一步证实了结节病中已知的炎症成分。miR-34a水平的变化可能表明其参与了该疾病的病理过程。
