Souza A, Cruz J C, Sousa N R, Procópio A R L, Silva G F
Laboratório de Biotecnologia e Recursos Naturais, Universidade do Estado do Amazonas, Manaus, AM, Brasil.
Laboratório de Biologia Molecular, Embrapa Amazônia Ocidental, Manaus, AM, Brasil.
Genet Mol Res. 2014 Oct 27;13(4):8661-70. doi: 10.4238/2014.October.27.6.
Endophytic microorganisms consist of fungi, bacteria, and actinomycetes that play important roles in the process of plant adaptation to the environment. Currently, the natural associations between microorganisms and plant species are being explored for a large number of biotechnological applications. In this study, 122 endophytic bacteria were isolated from 5 cultivars of Musa spp from the state of Amazonas (Brazil). Four strains were selected because they exhibited antagonistic activities against Fusarium oxysporum f. sp cubense and Colletotrichum guaranicola, with inhibitions ranging from 19 to 30% and 27 to 35%, respectively. Phylogenetic analysis of the 16S rDNA regions of these bacteria with antifungal activity showed that they are phylogenetically related to 3 different species of Bacillus - B. amyloliquefaciens, B. subtilis subsp subtilis, and B. thuringiensis.
内生微生物由真菌、细菌和放线菌组成，它们在植物适应环境的过程中发挥着重要作用。目前，正在探索微生物与植物物种之间的自然关联，以用于大量生物技术应用。在本研究中，从巴西亚马孙州的5个香蕉品种中分离出122株内生细菌。选择了4株菌株，因为它们对尖孢镰刀菌古巴专化型和瓜拉尼炭疽菌表现出拮抗活性，抑制率分别为19%至30%和27%至35%。对这些具有抗真菌活性的细菌的16S rDNA区域进行系统发育分析表明，它们在系统发育上与芽孢杆菌属的3个不同物种——解淀粉芽孢杆菌、枯草芽孢杆菌枯草亚种和苏云金芽孢杆菌相关。
