Ma Y, Guo J W, Bade R, Men Z H, Hasi A
Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, College of Life Sciences, Inner Mongolia University, Hohhot, China.
Department of Biological Science and Technology, Baotou Teacher's College, Baotou, China.
Genet Mol Res. 2014 Oct 27;13(4):8794-806. doi: 10.4238/2014.October.27.21.
The SBP-box gene family is specific to plants and encodes a class of zinc finger-containing transcription factors with a broad range of functions. Although SBP-box genes have been identified in numerous plants, including green algae, moss, silver birch, snapdragon, Arabidopsis, rice, and maize, there is little information concerning SBP-box genes, or the corresponding miR156/157, function in melon. Using the highly conserved sequence of the Arabidopsis thaliana SBP-box domain protein as a probe of information sequence, the genome-wide protein database of melon was explored to obtain 13 SBP-box protein sequences, which were further divided into 4 groups, based on phylogenetic analysis. A further analysis centered on the melon SBP-box genetic family's phylogenetic evolution, sequence similarities, gene structure, and miR156 target sequence was also conducted. Analysis of all the expression patterns of melon SBP-box family genes showed that the SBP-box genes were detected in 7 kinds of tissue, and fruit had the highest expression level. CmSBP11 tends to present its specific expression in melon fruit and root. CmSBP09 expression was the highest in flower. Overall, the molecular evolution and expression pattern of the melon SBP-box gene family, revealed by these results, suggest its function differentiation that followed gene duplication.
SBP-box基因家族是植物特有的，编码一类具有广泛功能的含锌指转录因子。尽管已在许多植物中鉴定出SBP-box基因，包括绿藻、苔藓、白桦、金鱼草、拟南芥、水稻和玉米，但关于甜瓜中SBP-box基因或相应的miR156/157的功能，所知甚少。以拟南芥SBP-box结构域蛋白的高度保守序列作为信息序列探针，对甜瓜的全基因组蛋白质数据库进行探索，获得了13个SBP-box蛋白序列，基于系统发育分析，这些序列进一步分为4组。还对甜瓜SBP-box基因家族的系统发育进化、序列相似性、基因结构和miR156靶序列进行了深入分析。对甜瓜SBP-box家族基因所有表达模式的分析表明，在7种组织中均检测到了SBP-box基因，其中果实中的表达水平最高。CmSBP11倾向于在甜瓜果实和根中呈现特异性表达。CmSBP09在花中的表达最高。总体而言，这些结果揭示的甜瓜SBP-box基因家族的分子进化和表达模式表明，其功能分化是在基因复制之后发生的。
