Ali Hina, Liu Yanhui, Azam Syed Muhammad, Rahman Zia Ur, Priyadarshani S V G N, Li Weimin, Huang Xinyu, Hu Bingyan, Xiong Junjie, Ali Umair, Qin Yuan
Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops; Center for Genomics and Biotechnology; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province 350002, China.
College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Int J Genomics. 2017;2017:1032846. doi: 10.1155/2017/1032846. Epub 2017 Sep 29.
Gene expression is regulated by transcription factors, which play many significant developmental processes. SQUAMOSA promoter-binding proteins (SBP) perform a variety of regulatory functions in leaf, flower, and fruit development, plant architecture, and sporogenesis. 16 genes were identified in pineapple and were divided into four groups on basis of phylogenetic analysis. Five paralogs in pineapple for genes were identified with Ka/Ks ratio varied from 0.20 for and to 0.36 for and , respectively. 16 genes were located on 12 chromosomes out of 25 pineapple chromosomes with highly conserved protein sequence structures. The isoionic points of SBP ranged from 6.05 to 9.57, while molecular weight varied from 22.7 to 121.9 kD. Expression profiles of genes revealed that and (leaf), , , , , , and (sepal), , , and (stamen), , , and (fruit) while the rest of genes showed low expression in studied tissues. Four genes, that is, , , , and , were highly expressed at 4°C, while were upregulated at 45°C. RNA-Seq was validated through qRT-PCR for some genes. Salt stress-induced expression of two genes, that is, and , while in drought stress, and were highly expressed. Our study lays a foundation for further gene function and expression studies of genes in pineapple.
基因表达受转录因子调控，转录因子在许多重要的发育过程中发挥作用。SQUAMOSA启动子结合蛋白（SBP）在叶片、花朵和果实发育、植物结构以及孢子发生中发挥多种调控功能。在菠萝中鉴定出16个基因，并根据系统发育分析将其分为四组。菠萝中鉴定出5个基因的旁系同源物，其Ka/Ks比值分别从和的0.20到和的0.36不等。16个基因位于25条菠萝染色体中的12条染色体上，具有高度保守的蛋白质序列结构。SBP的等电点范围为6.05至9.57，而分子量从22.7至121.9 kDa不等。基因的表达谱显示，和（叶片）、、、、、和（萼片）、、、和（雄蕊）、、、和（果实），而其余基因在研究组织中表达较低。四个基因，即、、和，在4°C时高表达，而在45°C时上调。通过qRT-PCR对一些基因验证了RNA-Seq。盐胁迫诱导两个基因，即和的表达，而在干旱胁迫下，和高表达。我们的研究为进一步研究菠萝中基因的功能和表达奠定了基础。
