Ribeiro Clarisse, Pärssinen Jenita, Sencadas Vítor, Correia Vítor, Miettinen S, Hytönen Vesa P, Lanceros-Méndez Senentxu
Center/Department of Physics, University of Minho, Braga, 4710-057, Portugal; INL-International Iberian Nanotechnology Laboratory, 4715-330, Braga, Portugal.
J Biomed Mater Res A. 2015 Jun;103(6):2172-5. doi: 10.1002/jbm.a.35368. Epub 2014 Nov 11.
This work reports on the influence of the substrate polarization of electroactive β-poly(vinylidene fluoride) (β-PVDF) on human adipose stem cells (hASCs) differentiation under static and dynamic conditions. hASCs were cultured on different β-PVDF surfaces (non-poled and "poled -") adsorbed with fibronectin and osteogenic differentiation was determined using a quantitative alkaline phosphatase assay. "Poled -" β-PVDF samples promote higher osteogenic differentiation, which is even higher under dynamic conditions. It is thus demonstrated that electroactive membranes can provide the necessary electromechanical stimuli for the differentiation of specific cells and therefore will support the design of suitable tissue engineering strategies, such as bone tissue engineering.
这项工作报道了电活性β-聚偏二氟乙烯（β-PVDF）的基底极化对静态和动态条件下人脂肪干细胞（hASCs）分化的影响。将hASCs培养在吸附有纤连蛋白的不同β-PVDF表面（未极化和“极化-”）上，并使用定量碱性磷酸酶测定法确定成骨分化情况。“极化-”β-PVDF样品促进更高的成骨分化，在动态条件下甚至更高。因此证明，电活性膜可为特定细胞的分化提供必要的机电刺激，从而将支持合适的组织工程策略的设计，例如骨组织工程。
