Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.
ICVS/3B's-PT Government Associate Laboratory, Braga, Portugal.
J Biomed Mater Res A. 2023 Jan;111(1):35-44. doi: 10.1002/jbm.a.37443. Epub 2022 Sep 7.
Electroactive smart materials play an important role for tissue regenerative applications. Poly(vinylidene fluoride) (PVDF) is a specific subtype of piezoelectric electroactive material that generates electrical potential upon mechanical stimulation. This work focuses on the application of piezoelectric PVDF films for neural differentiation. Human neural precursor cells (hNPCs) are cultured on piezoelectric poled and non-poled β-PVDF films with or without a pre-coating step of poly-d-lysine and laminin (PDL/L). Subsequently, hNPCs differentiation into the neuronal lineage is assessed (MAP2 and DCX ) under static or dynamic (piezoelectric stimulation) culture conditions. The results demonstrate that poled and coated β-PVDF films induce neuronal differentiation under static culture conditions which is further enhanced with mechanical stimulation. In silico calculations of the electrostatic potential of different domains of laminin, highlight the high polarity of those domains, which shows a clear preference to interact with the varying surface electric field of the piezoelectric material under mechanical stimulation. These interactions might explain the higher neuronal differentiation induced by poled β-PVDF films pre-coated with PDL/L under dynamic conditions. Our results suggest that electromechanical stimuli, such as the ones induced by piezoelectric β-PVDF films, are suitable to promote neuronal differentiation and hold great promise for the development of neuroregenerative therapies.
电活性智能材料在组织再生应用中起着重要作用。聚偏二氟乙烯（PVDF）是一种特殊的压电电活性材料亚类，在机械刺激下会产生电势。本工作重点研究压电 PVDF 薄膜在神经分化中的应用。将人神经前体细胞（hNPC）培养在极化和未极化的β-PVDF 薄膜上，有或没有聚-d-赖氨酸和层粘连蛋白（PDL/L）的预涂层步骤。随后，在静态或动态（压电刺激）培养条件下评估 hNPC 向神经元谱系的分化（MAP2 和 DCX）。结果表明，极化和涂层的β-PVDF 薄膜在静态培养条件下诱导神经元分化，机械刺激进一步增强了这种分化。层粘连蛋白不同结构域静电势的计算表明，这些结构域具有很高的极性，它们明显倾向于在机械刺激下与压电材料不断变化的表面电场相互作用。这些相互作用可能解释了在动态条件下经 PDL/L 预涂层的极化β-PVDF 薄膜诱导更高的神经元分化。我们的结果表明，机电刺激，如压电β-PVDF 薄膜诱导的刺激，适合促进神经元分化，为神经再生治疗的发展带来了巨大的希望。
