Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Sci Rep. 2017 Feb 16;7:42572. doi: 10.1038/srep42572.
T1C-19, a newly developed transgenic cry1C rice line, expresses cry1C under the control of the maize ubiquitin promoter, and is highly resistant to lepidopteran pests of rice. Cyrtorhinus lividipennis is the major predator of the eggs and young nymphs of Nilaparvata lugens, which is the main non-target sap-sucking insect pest of Bt rice. C. lividipennis may be exposed to Cry1C protein, thus biosafety evaluations of transgenic cry1C rice on C. lividipennis should be conducted before the commercialization of T1C-19. In the current study, we tested the direct toxicity of elevated doses of Cry1C to C. lividipennis, effects of T1C-19 on the life-table parameters of C. lividipennis via preying planthoppers, and effects of T1C-19 on the population density and dynamics in rice fields. No detrimental effects on development, survival, female ratio and body weight of C. lividipennis were caused by direct exposure to elevated doses of the Cry1C protein or prey-mediated exposure to realistic doses of the protein. The population density and dynamics did not significantly differ between C. lividipennis in T1C-19 and non-transgenic rice fields. Thus, transgenic cry1C rice had no negative effects on C. lividipennis. This is the first report of the effects of transgenic cry1C rice on C. lividipennis.
T1C-19 是一个新开发的转 Cry1C 水稻品系，在玉米泛素启动子的控制下表达 Cry1C，对鳞翅目水稻害虫具有高度抗性。三化螟是褐飞虱卵和若虫的主要捕食者，褐飞虱是 Bt 水稻的主要非靶标刺吸式害虫。C. lividipennis 可能会接触到 Cry1C 蛋白，因此在 T1C-19 商业化之前，应该对转 Cry1C 水稻对 C. lividipennis 的生物安全性进行评估。在本研究中，我们测试了Cry1C 蛋白高剂量对 C. lividipennis 的直接毒性、T1C-19 通过捕食褐飞虱对 C. lividipennis 生命表参数的影响，以及 T1C-19 对稻田种群密度和动态的影响。直接暴露于高剂量 Cry1C 蛋白或通过捕食实际剂量的蛋白介导暴露于 C. lividipennis 的发育、生存、雌性比例和体重均未产生不利影响。T1C-19 和非转基因稻田中的 C. lividipennis 种群密度和动态没有显著差异。因此，转 Cry1C 水稻对 C. lividipennis 没有负面影响。这是转 Cry1C 水稻对 C. lividipennis 影响的首次报道。
