CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
ACS Nano. 2014 Nov 25;8(11):11715-23. doi: 10.1021/nn5051344. Epub 2014 Nov 10.
The structural arrangement of amino acid residues in a native enzyme provides a blueprint for the design of artificial enzymes. One challenge of mimicking the catalytic center of a native enzyme is how to arrange the essential amino acid residues in an appropriate position. In this study, we designed an artificial hydrolase via self-assembly of short peptides to catalyze ester hydrolysis. When the assembled hydrolase catalytic sites were embedded in a matrix of peptide nanofibers, they exhibited much higher catalytic efficiency than the peptide nanofibers without the catalytic sites, suggesting that this well-ordered nanostructure is an attractive scaffold for developing new artificial enzymes. Furthermore, the cytotoxicity of the assembled hydrolase was evaluated with human cells, and the novel artificial biological enzyme showed excellent biocompatibility.
天然酶中氨基酸残基的结构排列为人工酶的设计提供了蓝图。模拟天然酶催化中心的一个挑战是如何将必需的氨基酸残基排列在适当的位置。在这项研究中，我们通过短肽的自组装设计了一种人工水解酶来催化酯水解。当组装的水解酶催化位点嵌入肽纳米纤维基质中时，它们表现出比没有催化位点的肽纳米纤维更高的催化效率，这表明这种有序的纳米结构是开发新型人工酶的有吸引力的支架。此外，还用人细胞评估了组装水解酶的细胞毒性，新型人工生物酶表现出良好的生物相容性。
