Hashimoto H, Müller H, Müller F, Schmidts H L, Stutte H J
Senekenbergisches Zentrum der Pathologie, Universität Frankfurt am Main, Federal Republic of Germany.
Virchows Arch A Pathol Anat Histopathol. 1987;411(5):441-8. doi: 10.1007/BF00735225.
Cytomegalovirus (CMV) was assayed by in situ hybridization with commercially available biotin-labeled CMV-DNA probes in 45 formalin-fixed paraffin-embedded autopsy specimens with Kaposi's sarcoma from 14 cases of the acquired immune deficiency syndrome (AIDS). In seven of the 14 cases, a few scattered hybridizing cells were detected in Kaposi's sarcoma, but not all specimens from the same case showed such cells. Most of the positive cells were peculiarly swollen and not typical of Kaposi's sarcoma cells. All positive cases had at least some CMV-infected organs with typical cytomegalic cells containing nuclear inclusions while five of the 7 negative cases revealed no CMV-infected tissue by conventional light microscopy. Our results suggest that this in situ hybridization procedure using biotin-labeled DNA probes only reveals generalized CMV infection that is a consequence of impairment of immune mechanisms in AIDS patients.
采用市售生物素标记的巨细胞病毒（CMV）DNA探针，通过原位杂交法，对14例获得性免疫缺陷综合征（AIDS）患者的45份伴有卡波西肉瘤的福尔马林固定石蜡包埋尸检标本进行检测。在14例中的7例中，卡波西肉瘤内检测到少量散在的杂交细胞，但同一病例的所有标本并非均显示有此类细胞。大多数阳性细胞异常肿胀，并非典型的卡波西肉瘤细胞。所有阳性病例均至少有一些器官被CMV感染，有典型的含核内包涵体的巨细胞，而7例阴性病例中的5例经传统光学显微镜检查未发现CMV感染的组织。我们的结果表明，使用生物素标记DNA探针的这种原位杂交方法仅揭示了全身性CMV感染，这是AIDS患者免疫机制受损的结果。
