School of Engineering, École Polytechnique Fédérale de Lausanne , Lausanne 1015, Switzerland.
Sibley School of Mechanical and Aerospace Engineering, Cornell University , Ithaca, New York 14853, USA.
Biomicrofluidics. 2014 Jul 21;8(4):044108. doi: 10.1063/1.4890943. eCollection 2014 Jul.
We present a method to perform sample concentration within a lab-on-a-chip using a microfluidic structure which controls the liquid-gas interface through a micropillar array fabricated in polydimethylsiloxane between microfluidic channels. The microstructure confines the liquid flow and a thermal gradient is used to drive evaporation at the liquid-gas-interface. The evaporation occurs in-plane to the microfluidic device, allowing for precise control of the ambient environment. This method is demonstrated with a sample containing 1 μm, 100 nm fluorescent beads and SYTO-9 labelled Escherichia coli bacteria. Over 100 s, the fluorescent beads and bacteria are concentrated by a factor of 10.
我们提出了一种在微流控芯片内通过微流道之间的 PDMS 微柱阵列控制气液界面来进行样品浓缩的方法。该微结构限制了液体流动，利用热梯度在气液界面处驱动蒸发。蒸发是在平面上进行的，从而可以精确控制环境。该方法使用含有 1 μm、100nm 荧光珠和 SYTO-9 标记的大肠杆菌的样品进行了验证。在 100s 内，荧光珠和细菌的浓度提高了 10 倍。
