Department of Mechanical Engineering, University of Maryland , College Park, Maryland 20742, USA.
Department of Materials Science and Engineering, University of Maryland , College Park, Maryland 20742, USA.
Biomicrofluidics. 2014 Jul 21;8(4):044109. doi: 10.1063/1.4891100. eCollection 2014 Jul.
A technique for microfluidic, pH modulated DNA capture and purification using chitosan functionalized glycidyl methacrylate monoliths is presented. Highly porous polymer monoliths are formed and subsequently functionalized off-chip in a batch process before insertion into thermoplastic microchannels prior to solvent bonding, simplifying the overall fabrication process by eliminating the need for on-chip surface modifications. The monolith anchoring method allows for the use of large cross-section monoliths enabling high flowrates and high DNA capture capacity with a minimum of added design complexity. Using monolith capture elements requiring less than 1 mm(2) of chip surface area, loading levels above 100 ng are demonstrated, with DNA capture and elution efficiency of 54.2% ± 14.2% achieved.
本文介绍了一种使用壳聚糖功能化甲基丙烯酸缩水甘油酯整体柱的微流控、pH 调节 DNA 捕获和纯化技术。首先形成高度多孔的聚合物整体柱，然后在非芯片批次工艺中进行功能化，之后在热塑性微通道中插入之前进行溶剂键合，通过消除对芯片表面修饰的需求，简化了整体制造工艺。整体柱固定方法允许使用大截面整体柱，从而实现高流速和高 DNA 捕获能力，同时设计复杂度最小。使用所需芯片表面积小于 1mm²的整体柱捕获元件，可实现超过 100ng 的上载水平，实现 54.2%±14.2%的 DNA 捕获和洗脱效率。
