用于灌注细胞培养的具有三维相互连通微孔壁的聚苯乙烯基微流控装置。
A polystyrene-based microfluidic device with three-dimensional interconnected microporous walls for perfusion cell culture.
机构信息
Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, USA.
Science & Technology, Corning Incorporated , Corning, New York 14831, USA.
出版信息
Biomicrofluidics. 2014 Aug 27;8(4):046505. doi: 10.1063/1.4894409. eCollection 2014 Jul.
Abstract
In this article, we present a simple, rapid prototyped polystyrene-based microfluidic device with three-dimensional (3D) interconnected microporous walls for long term perfusion cell culture. Patterned 3D interconnected microporous structures were created by a chemical treatment together with a protective mask and the native hydrophobic nature of the microporous structures were selectively made hydrophilic using oxygen plasma treatment together with a protective mask. Using this polystyrene-based cell culture microfluidic device, we successfully demonstrated the support of four days perfusion cell culture of hepatocytes (C3A cells).
摘要
本文提出了一种简单、快速原型化的基于聚苯乙烯的微流控装置，具有三维（3D）互连的微孔壁，用于长期灌注细胞培养。通过化学处理、保护罩以及微孔结构的固有疏水性，共同创造出具有图案的 3D 互连微孔结构，然后使用氧气等离子体处理和保护罩，选择性地将其亲水化。使用这种基于聚苯乙烯的细胞培养微流控装置，我们成功地演示了支持肝实质细胞（C3A 细胞）四天灌注培养的能力。
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