Sherkat Roya, Yazdani Reza, Ganjalikhani Hakemi Mazdak, Homayouni Vida, Farahani Rahim, Hosseini Mohsen, Rezaei Abbas
Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Immunology Department, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Asia Pac Allergy. 2014 Oct;4(4):212-21. doi: 10.5415/apallergy.2014.4.4.212. Epub 2014 Oct 29.
In this study, the expression of interleukin-9 (IL-9), IL-17, IL-22, and IL-25 genes that might be the potential predisposing factors for asthma as well as count of innate lymphoid cells (ILCs) as another source of inflammatory cytokines have been evaluated.
The aim of this study was to evaluate the expression of newly identified helper T cells signature cytokines and amount of ILCs.
Blood and sputum samples from 23 patients with moderate to severe asthma and 23 healthy volunteers were collected. The types of allergens to which our patients were sensitive were defined using immunoblotting method. Gene expression of studied cytokines was evaluated using quantitative transcription-polymerase chain reaction and ILCs were counted by the flow cytometry method.
In this research, the gene expressions of IL-9, IL-17, IL-22, and IL-25 were significantly higher in asthmatics, especially in the severe form of the disease. This increase was even higher in serum samples compared with sputum samples. Counting ILCs revealed their increase in comparison with normal people.
We showed the importance of IL-25, IL-22, IL-17, and IL-9 cytokines in patients with asthma as their expression levels are increased and these increase are correlated with the severity of the disease. We also showed that the increased amount of ILCs in asthmatics could confirm their potential role in the immunopathogenesis of asthma as another source of inflammatory cytokines.
在本研究中，评估了白细胞介素-9（IL-9）、IL-17、IL-22和IL-25基因的表达，这些基因可能是哮喘的潜在易感因素，同时还评估了作为炎症细胞因子另一个来源的天然淋巴细胞（ILC）数量。
本研究的目的是评估新发现的辅助性T细胞标志性细胞因子的表达以及ILC的数量。
收集了23例中重度哮喘患者和23名健康志愿者的血液和痰液样本。使用免疫印迹法确定患者敏感的过敏原类型。采用定量转录-聚合酶链反应评估所研究细胞因子的基因表达，并通过流式细胞术对ILC进行计数。
在本研究中，哮喘患者中IL-9、IL-17、IL-22和IL-25的基因表达显著更高，尤其是在疾病的重度形式中。与痰液样本相比，血清样本中的这种增加更为明显。对ILC的计数显示，与正常人相比其数量增加。
我们表明IL-25、IL-22、IL-17和IL-9细胞因子在哮喘患者中具有重要意义，因为它们的表达水平升高，且这些升高与疾病的严重程度相关。我们还表明，哮喘患者中ILC数量的增加证实了它们作为炎症细胞因子的另一个来源在哮喘免疫发病机制中的潜在作用。
