Galès Amandine, Latrille Eric, Wéry Nathalie, Steyer Jean-Philippe, Godon Jean-Jacques
INRA, UR0050, Laboratoire de Biotechnologie de l'Environnement, Narbonne, France.
PLoS One. 2014 Nov 7;9(11):e112182. doi: 10.1371/journal.pone.0112182. eCollection 2014.
We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.
我们建议利用松树针叶表面对堆肥厂的生物气溶胶排放进行生物监测。测量基于直丝糖多孢菌的16S rRNA基因拷贝数，该菌是堆肥厂排放的生物指示物。基于排放源周围的29个样本制定了采样计划。每克阿勒颇松针叶中直丝糖多孢菌16S rRNA基因拷贝数的丰度随距离变化，范围从104到102。根据当地地形和平均风向，在堆肥厂周围2公里至超过5.4公里的距离处，信号达到背景水平。根据这些数值，绘制了生物气溶胶源周围的受影响区域图。
