Tang Haoyu, Yin Lichen, Kim Kyung Hoon, Cheng Jianjun
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL, 61801, USA.
Chem Sci. 2013 Oct;4(10):3839-3844. doi: 10.1039/C3SC51328A.
Poly(arginine) mimics bearing long hydrophobic side chains adopt stable helical conformation and exhibit helix-related cell-penetrating properties. Elongating polypeptide backbone length and increasing side chain hydrophobicity further increase the helicities of poly(arginine) mimics. They show superior cell membrane permeability up to two orders of magnitude higher than that of HIV-TAT peptide and excellent DNA and siRNA delivery efficiencies in various mammalian cells.
带有长疏水侧链的聚精氨酸模拟物具有稳定的螺旋构象,并表现出与螺旋相关的细胞穿透特性。延长多肽主链长度和增加侧链疏水性会进一步提高聚精氨酸模拟物的螺旋度。它们表现出比HIV-TAT肽高两个数量级的卓越细胞膜通透性,以及在各种哺乳动物细胞中出色的DNA和siRNA递送效率。