Martín-Mateos Pedro, Crespo-Garcia Sergio, Ruiz-Llata Marta, Lopez-Fernandez José Ramón, Jorcano José Luis, Del Rio Marcela, Larcher Fernando, Acedo Pablo
Department of Electronics Technology, Universidad Carlos III de Madrid, Leganes, Madrid 28911, Spain.
Epithelial Biomedicine Division, CIEMAT, Avenida Complutense 40, Madrid 28040, Spain and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, Spain.
Biomed Opt Express. 2014 Aug 28;5(9):3231-7. doi: 10.1364/BOE.5.003231. eCollection 2014 Sep 1.
In this study the first results on evaluation and assessment of grafted bioengineered skin substitutes using an optical Diffuse Reflectance Spectroscopy (DRS) system with a remote optical probe are shown. The proposed system is able to detect early vascularization of skin substitutes expressing the Vascular Endothelial Growth Factor (VEGF) protein compared to normal grafts, even though devitalized skin is used to protect the grafts. Given the particularities of the biological problem, data analysis is performed using two Blind Signal Separation (BSS) methods: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). These preliminary results are the first step towards point-of-care diagnostics for skin implants early assessment.
本研究展示了使用带有远程光学探头的光学漫反射光谱（DRS）系统对移植的生物工程皮肤替代品进行评估和评价的首批结果。与正常移植物相比，该系统能够检测出表达血管内皮生长因子（VEGF）蛋白的皮肤替代品的早期血管形成，即便使用失活皮肤来保护移植物。鉴于该生物学问题的特殊性，使用两种盲信号分离（BSS）方法进行数据分析：主成分分析（PCA）和独立成分分析（ICA）。这些初步结果是朝着皮肤植入物早期评估的即时诊断迈出的第一步。
