Chi Kai-Hua, Danavall Damien, Taleo Fasihah, Pillay Allan, Ye Tun, Nachamkin Eli, Kool Jacob L, Fegan David, Asiedu Kingsley, Vestergaard Lasse S, Ballard Ronald C, Chen Cheng-Yen
Laboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines.
Laboratory Reference and Research Branch, Division of STD Prevention, National Center for HIV/AIDS, Viral Hepatitis, STD and Tuberculosis Prevention, Atlanta, Georgia; Centers for Disease Control and Prevention, Atlanta, Georgia; Ministry of Health, Port Vila, Vanuatu; Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization, Port Vila, Vanuatu; World Health Organization Consultant, Springhill, Brisbane, Australia; Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland; World Health Organization, Western Pacific Regional Office, Manila, Philippines
Am J Trop Med Hyg. 2015 Jan;92(1):134-8. doi: 10.4269/ajtmh.14-0459. Epub 2014 Nov 17.
We developed a TaqMan-based real-time quadriplex polymerase chain reaction (PCR) to simultaneously detect Treponema pallidum subspecies pallidum, T. pallidum subsp. pertenue, and T. pallidum subsp. endemicum, the causative agents of venereal syphilis, yaws, and bejel, respectively. The PCR assay was applied to samples from skin ulcerations of clinically presumptive yaws cases among children on Tanna Island, Vanuatu. Another real-time triplex PCR was used to screen for the point mutations in the 23S rRNA genes that have previously been associated with azithromycin resistance in T. pallidum subsp. pallidum strains. Seropositivity by the classical syphilis serological tests was 35.5% among children with skin ulcerations clinically suspected with yaws, whereas the presence of T. pallidum subsp. pertenue DNA was only found in lesions from 15.5% of children. No evidence of T. pallidum subsp. pertenue infection, by either PCR or serology was found in ∼59% of cases indicating alternative causes of yaws-like lesions in this endemic area.
我们开发了一种基于TaqMan的实时四重聚合酶链反应（PCR），用于同时检测梅毒螺旋体苍白亚种、雅司螺旋体和地方螺旋体，它们分别是性病梅毒、雅司病和地方性梅毒的病原体。该PCR检测方法应用于来自瓦努阿图塔纳岛临床疑似雅司病儿童皮肤溃疡的样本。另一种实时三重PCR用于筛查23S rRNA基因中的点突变，这些突变先前已与梅毒螺旋体苍白亚种菌株中的阿奇霉素耐药性相关。在临床疑似雅司病的皮肤溃疡儿童中，经典梅毒血清学检测的血清阳性率为35.5%，而仅在15.5%儿童的病变中发现了雅司螺旋体DNA。在约59%的病例中，未发现通过PCR或血清学检测的雅司螺旋体感染证据，这表明该流行地区存在类似雅司病病变的其他病因。
