用于从人血液中检测、分离和培养活肿瘤细胞的纳米荧光探针。
NanoFlares for the detection, isolation, and culture of live tumor cells from human blood.
作者信息
Halo Tiffany L, McMahon Kaylin M, Angeloni Nicholas L, Xu Yilin, Wang Wei, Chinen Alyssa B, Malin Dmitry, Strekalova Elena, Cryns Vincent L, Cheng Chonghui, Mirkin Chad A, Thaxton C Shad
机构信息
Department of Chemistry, International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208-3113;
Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL 60611; Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, Chicago, IL, 60611; Walter S. and Lucienne Driskill Graduate Training Program in Life Sciences, Northwestern University, Chicago, IL 60611;
出版信息
Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17104-9. doi: 10.1073/pnas.1418637111. Epub 2014 Nov 17.
Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood—so-called circulating tumor cells (CTCs)—may provide unprecedented opportunities for metastatic risk assessment and investigation. NanoFlares are nanoconstructs that enable live-cell detection of intracellular mRNA. NanoFlares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, NanoFlares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.
转移预示着癌症患者的预后不良。在可检测到的转移病灶出现之前，原发性肿瘤细胞就通过血液循环扩散。对血液中癌细胞（即所谓的循环肿瘤细胞，CTCs）的分析可能为转移风险评估和研究提供前所未有的机会。纳米荧光探针是一种纳米结构，能够对活细胞内的mRNA进行检测。当纳米荧光探针与流式细胞术结合使用时，可用于在全血环境中对CTCs的遗传标记进行荧光检测。它们能够检测出每毫升血液中低至100个活癌细胞，并随后对这些细胞进行培养。该技术还可用于在转移性乳腺癌小鼠模型中检测CTCs。因此，据我们所知，纳米荧光探针提供了第一种基于基因的方法，用于从血液中检测、分离和鉴定活癌细胞，并可能为癌症诊断、预后和个性化治疗提供新的机会。
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