Ahluwalia Balpreet Singh, McCourt Peter, Oteiza Ana, Wilkinson James S, Huser Thomas R, Hellesø Olav Gaute
Department of Physics and Technology, University of Tromsø, N-9037 Tromsø, Norway.
Analyst. 2015 Jan 7;140(1):223-9. doi: 10.1039/c4an01181c.
Red blood cells squeeze through micro-capillaries as part of blood circulation in the body. The deformability of red blood cells is thus critical for blood circulation. In this work, we report a method to optically squeeze red blood cells using the evanescent field present on top of a planar waveguide chip. The optical forces from a narrow waveguide are used to squeeze red blood cells to a size comparable to the waveguide width. Optical forces and pressure distributions on the cells are numerically computed to explain the squeezing process. The proposed technique is used to quantify the loss of blood deformability that occurs during blood storage lesion. Squeezing red blood cells using waveguides is a sensitive technique and works simultaneously on several cells, making the method suitable for monitoring stored blood.
红细胞在体内血液循环过程中挤过微毛细血管。因此，红细胞的可变形性对于血液循环至关重要。在这项工作中，我们报告了一种利用平面波导芯片顶部存在的倏逝场来光学挤压红细胞的方法。来自窄波导的光学力被用于将红细胞挤压到与波导宽度相当的尺寸。对细胞上的光学力和压力分布进行了数值计算，以解释挤压过程。所提出的技术用于量化血液储存损伤期间发生的血液可变形性损失。利用波导挤压红细胞是一种灵敏的技术，可同时作用于多个细胞，使得该方法适用于监测储存的血液。
