CCL17和CCL18在结核样型和瘤型麻风病中的皮肤差异表达
Differential dermal expression of CCL17 and CCL18 in tuberculoid and lepromatous leprosy.
作者信息
Berrington William R, Kunwar Chhatra B, Neupane Kapil, van den Eeden Susan J F, Vary James C, Peterson Glenna J, Wells Richard D, Geluk Annemieke, Hagge Deanna A, Hawn Thomas R
机构信息
University of Washington School of Medicine, Seattle, Washington, United States of America.
Mycobacterial Research Laboratory, Anandaban Hospital, Kathmandu, Nepal.
出版信息
PLoS Negl Trop Dis. 2014 Nov 20;8(11):e3263. doi: 10.1371/journal.pntd.0003263. eCollection 2014.
BACKGROUND
Leprosy is characterized by polar clinical, histologic and immunological presentations. Previous immunologic studies of leprosy polarity were limited by the repertoire of cytokines known at the time.
METHODOLOGY
We used a candidate gene approach to measure mRNA levels in skin biopsies from leprosy lesions. mRNA from 24 chemokines and cytokines, and 6 immune cell type markers were measured from 85 Nepalese leprosy subjects. Selected findings were confirmed with immunohistochemistry.
PRINCIPAL RESULTS
Expression of three soluble mediators (CCL18, CCL17 and IL-10) and one macrophage cell type marker (CD14) was significantly elevated in lepromatous (CCL18, IL-10 and CD14) or tuberculoid (CCL17) lesions. Higher CCL18 protein expression by immunohistochemistry and a trend in increased serum CCL18 in lepromatous lesions was observed. No cytokines were associated with erythema nodosum leprosum or Type I reversal reaction following multiple comparison correction. Hierarchical clustering suggested that CCL18 was correlated with cell markers CD209 and CD14, while neither CCL17 nor CCL18 were highly correlated with classical TH1 and TH2 cytokines.
CONCLUSIONS
Our findings suggest that CCL17 and CCL18 dermal expression is associated with leprosy polarity.
背景
麻风病具有两极分化的临床、组织学和免疫学表现。以往对麻风病两极分化的免疫学研究受到当时已知细胞因子种类的限制。
方法
我们采用候选基因方法来测量麻风病皮损皮肤活检组织中的mRNA水平。对85名尼泊尔麻风病患者的24种趋化因子和细胞因子以及6种免疫细胞类型标志物的mRNA进行了检测。选定的结果通过免疫组织化学得到证实。
主要结果
三种可溶性介质（CCL18、CCL17和IL-10）和一种巨噬细胞类型标志物（CD14）在瘤型（CCL18、IL-10和CD14）或结核样型（CCL17）皮损中的表达显著升高。通过免疫组织化学观察到瘤型皮损中CCL18蛋白表达更高，且血清CCL18有升高趋势。经多重比较校正后，未发现细胞因子与麻风结节性红斑或I型反应逆转相关。层次聚类分析表明CCL18与细胞标志物CD209和CD14相关，而CCL17和CCL18均与经典的TH1和TH2细胞因子高度不相关。
结论
我们的研究结果表明CCL17和CCL18在皮肤中的表达与麻风病的两极分化有关。
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